Cunningham J J, Lydon M K, Emerson R, Harmatz P R
Shriners Burns Institute, Massachusetts General Hospital, Boston, USA.
Nutrition. 1996 Feb;12(2):83-8. doi: 10.1016/0899-9007(96)90704-2.
Copper (Cu) status is often judged by the plasma level of its chief transport protein, ceruloplasmin (Cp). Only copper deficiency and heredity are known to decrease circulating Cp. Cp is an acute-phase responsive protein in trauma and it is also induced by Cu supplementation. Despite this, plasma concentrations of Cp remain low during the acute recovery from major burn injury. The high provision of vitamin C typically used in burn patients may influence these observations when an indirect oxidase activity assay is used. We employed a radial immunodiffusion (RID) assay specific for the Cp protein as well as an indirect oxidase assay for Cp in a series of 11 burned children who were supplemented with both Cu and vitamin C, either enterally or parenterally. Our findings confirm that low Cp is a characteristic of the acute recovery from major burns. The oxidase assay is shown to be valid for very low Cp levels even during high vitamin C provision. When these data are combined with our previously reported series, a strong relationship between the size of the open wound area and the amount of circulating Cp is demonstrated. Copper supplementation by either the enteral or parenteral routes is only marginally successful in restoring Cp toward normal levels.
铜（Cu）状态通常通过其主要转运蛋白铜蓝蛋白（Cp）的血浆水平来判断。已知只有铜缺乏和遗传因素会降低循环中的Cp水平。Cp是创伤中的一种急性期反应蛋白，补充铜也可诱导其产生。尽管如此，在严重烧伤后的急性恢复期，血浆中Cp的浓度仍然很低。当使用间接氧化酶活性测定法时，烧伤患者通常大量补充的维生素C可能会影响这些观察结果。我们对11名烧伤儿童进行了一系列研究，这些儿童通过肠内或肠外途径同时补充了铜和维生素C，我们采用了针对Cp蛋白的放射免疫扩散（RID）测定法以及Cp的间接氧化酶测定法。我们的研究结果证实，低Cp是严重烧伤急性恢复期的一个特征。即使在大量补充维生素C的情况下，氧化酶测定法对于极低的Cp水平也是有效的。当将这些数据与我们之前报道的系列研究相结合时，开放性伤口面积大小与循环中Cp含量之间呈现出很强的相关性。通过肠内或肠外途径补充铜，在使Cp恢复到正常水平方面仅取得了有限的成功。
