烟草线条病毒RNA 3的完整核苷酸序列
Complete nucleotide sequence of tobacco streak virus RNA 3.
作者信息
Cornelissen B J, Janssen H, Zuidema D, Bol J F
出版信息
Nucleic Acids Res. 1984 Mar 12;12(5):2427-37. doi: 10.1093/nar/12.5.2427.
Abstract
Double-stranded cDNA of in vitro polyadenylated tobacco streak virus (TSV) RNA 3 has been cloned and sequenced. The complete primary structure of 2,205 nucleotides reveals two open reading frames flanked by a leader sequence of 210 bases, an intercistronic region of 123 nucleotides and a 3'-extracistronic sequence of 288 nucleotides. The 5'-terminal open reading frame codes for a Mr 31,742 protein, which probably corresponds to the only in vitro translation product of TSV RNA 3. The 3'-terminal coding region predicts a Mr 26,346 protein, probably the viral coat protein, which is the translation product of the subgenomic messenger, RNA 4. Although the coat proteins of alfalfa mosaic virus (A1MV) and TSV are functionally equivalent in activating their own and each others genomes, no homology between the primary structures of those two proteins is detectable.
摘要
体外聚腺苷酸化的烟草条纹病毒（TSV）RNA 3的双链cDNA已被克隆并测序。2205个核苷酸的完整一级结构显示有两个开放阅读框，两侧分别是210个碱基的前导序列、123个核苷酸的顺反子间区域和288个核苷酸的3'-顺反子外序列。5'-末端开放阅读框编码一种分子量为31742的蛋白质，它可能对应于TSV RNA 3唯一的体外翻译产物。3'-末端编码区预测有一个分子量为26346的蛋白质，可能是病毒外壳蛋白，它是亚基因组信使RNA 4的翻译产物。尽管苜蓿花叶病毒（A1MV）和TSV的外壳蛋白在激活它们自身及彼此的基因组方面功能相当，但这两种蛋白质的一级结构之间未检测到同源性。
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