Zheng L, Benedict M Q, Cornel A J, Collins F H, Kafatos F C
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Genetics. 1996 Jun;143(2):941-52. doi: 10.1093/genetics/143.2.941.
We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitos from 13 backcross families that included 679 progeny. Three linkage groups were identified, corresponding to the three chromosomes. We added 22 new markers to the existing X chromosome map, for a total of 46 microsatellite markers spanning a distance of 48.9 cM. The second chromosome has 57 and the third 28 microsatellite markers spanning a distance of 72.4 and 93.7 cM, respectively. The overall average distance between markers is 1.6 cM (or 1.1, 1.2, and 3.2 cM for the X, second, and third chromosomes, respectively). In addition to the 131 microsatellite markers, the current map also includes a biochemical selectable markers, Dieldrin resistance (Dl), on the second chromosome and five visible markers, pink-eye (p) and white (w) on the X, collarless (c) and lunate (lu) on the second, and red-eye (r) on the third. The cytogenetic locations on the nurse cell polytene chromosomes have been determined for 47 markers, making this map an integrated tool for cytogenetic, genetic, and molecular analysis.
我们展示了基于微卫星多态性构建的非洲人类疟疾媒介冈比亚按蚊的遗传图谱。在实验室菌株中，89%的测试微卫星标记检测到多态性。对来自13个回交家系的679只子代个体蚊子进行了基因分型。鉴定出三个连锁群，对应于三条染色体。我们在现有的X染色体图谱上添加了22个新标记，共有46个微卫星标记，跨度为48.9厘摩。第二条染色体有57个微卫星标记，跨度为72.4厘摩，第三条染色体有28个微卫星标记，跨度为93.7厘摩。标记之间的总体平均距离为1.6厘摩（X染色体、第二条染色体和第三条染色体分别为1.1、1.2和3.2厘摩）。除了131个微卫星标记外，当前图谱还包括一个生化选择标记，即第二条染色体上的狄氏剂抗性（Dl），以及五个可见标记，X染色体上的粉红眼（p）和白眼（w），第二条染色体上的无领（c）和新月形（lu），第三条染色体上的红眼（r）。已确定47个标记在滋养细胞多线染色体上的细胞遗传学位置，使该图谱成为细胞遗传学、遗传学和分子分析的综合工具。
