冈比亚按蚊的综合遗传图谱：利用随机扩增多态性DNA（RAPD）多态性构建遗传、细胞遗传和序列标签位点（STS）标记
Integrated genetic map of Anopheles gambiae: use of RAPD polymorphisms for genetic, cytogenetic and STS landmarks.
作者信息
Dimopoulos G, Zheng L, Kumar V, della Torre A, Kafatos F C, Louis C
机构信息
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece.
出版信息
Genetics. 1996 Jun;143(2):953-60. doi: 10.1093/genetics/143.2.953.
Abstract
Randomly amplified polymorphic DNA (RAPD) markers have been integrated in the genetic and cytogenetic maps of the malaria vector mosquito, Anopheles gambiae. Fifteen of these markers were mapped by recombination, relative to microsatellite markers that had been mapped previously. Thirty-four gel-purified RAPD bands were cloned and sequenced, generating sequence tagged sites (STSs) that can be used as entry points to the A. gambiae genome. Thirty one of these STSs were localized on nurse cell polytene chromosomes through their unique hybridization signal in in situ hybridization experiments. Five STSs map close to the breakpoints of polymorphic inversions, which are notable features of the Anopheles genome. The usefulness and limitations of this integrated mosquito map are discussed.
摘要
随机扩增多态性DNA（RAPD）标记已被整合到疟疾媒介蚊虫冈比亚按蚊的遗传图谱和细胞遗传图谱中。相对于先前已定位的微卫星标记，其中15个标记通过重组进行了定位。34条凝胶纯化的RAPD条带被克隆并测序，产生了可作为进入冈比亚按蚊基因组切入点的序列标签位点（STS）。通过原位杂交实验中它们独特的杂交信号，其中31个STS定位在滋养细胞多线染色体上。5个STS定位在多态性倒位的断点附近，这是按蚊基因组的显著特征。本文讨论了这一整合的蚊虫图谱的实用性和局限性。
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