Fernández-Caldas E, Puerta L, Caraballo L, Mercado D, Lockey R F
Division of Allergy and Immunology, University of South Florida College of Medicine, Tampa, USA.
J Investig Allergol Clin Immunol. 1996 Mar-Apr;6(2):98-102.
The purpose of this study was to establish seasonal levels of Der p 1, Der f 1 and D. pteronyssinus allergens in mattress and floor dust samples in the homes of 20 mite-allergic asthmatic patients in Cartagena, Colombia. Dust samples were collected using a portable vacuum cleaner once monthly for the same 12 months in all houses. The highest Der p 1 levels in mattress samples were detected in August (geometric mean = 109.49 ng/g) and represented a 2.2-times increase from the lowest level observed in November. Der f 1 was detected in only 3 mattresses, ranging from 90 ng to 1.6 micrograms per gram of dust. The highest levels were detected in February and March. The highest level of D. pteronyssinus allergens in mattress dust samples, quantitated by RAST-inhibition, was 8, 994 AU/g (80% inhibition); the lowest level detected was 150 AU/g (5% inhibition). Allergen levels were significantly higher in mattresses than in floor samples (p < 0.001). Overall, a Spearman rank correlation coefficient of 0.48, p < 0.001, was obtained between Der p 1 and D. pteronyssinus allergens. There was a good correlation between mite allergens and absolute humidity (r = 0.8, p = 0.007). Der p 1, Der f 1 and D. pteronyssinus allergen levels show minimal variations in this tropical environment. Allergens derived from various mite species must be considered when assessing mite allergen exposure in these environments.
本研究的目的是确定哥伦比亚卡塔赫纳20名对螨过敏的哮喘患者家中床垫和地板灰尘样本中Der p 1、Der f 1和粉尘螨过敏原的季节性水平。在所有房屋中，每月使用便携式吸尘器收集一次灰尘样本，持续12个月。床垫样本中Der p 1的最高水平在8月被检测到（几何平均值 = 109.49 ng/g），比11月观察到的最低水平增加了2.2倍。仅在3个床垫中检测到Der f 1，每克灰尘含量在90 ng至1.6微克之间。最高水平在2月和3月被检测到。通过RAST抑制法测定，床垫灰尘样本中粉尘螨过敏原的最高水平为8994 AU/g（80%抑制）；检测到的最低水平为150 AU/g（5%抑制）。床垫中的过敏原水平显著高于地板样本（p < 0.001）。总体而言，Der p 1与粉尘螨过敏原之间的Spearman等级相关系数为0.48，p < 0.001。螨过敏原与绝对湿度之间存在良好的相关性（r = 0.8，p = 0.007）。在这个热带环境中，Der p 1、Der f 1和粉尘螨过敏原水平显示出最小的变化。在评估这些环境中的螨过敏原暴露时，必须考虑来自各种螨种的过敏原。
