采用杂交保护分析法对结核分枝杆菌进行新药敏感性试验。
New drug susceptibility test for Mycobacterium tuberculosis using the hybridization protection assay.
作者信息
Miyamoto J, Koga H, Kohno S, Tashiro T, Hara K
机构信息
Second Department of Internal Medicine, Nagasaki University, School of Medicine, Japan.
出版信息
J Clin Microbiol. 1996 May;34(5):1323-6. doi: 10.1128/jcm.34.5.1323-1326.1996.
Abstract
We developed a novel method for early detection of drug-resistant strains of Mycobacterium tuberculosis by using the hybridization protection assay (HPA). The number of viable bacteria during the incubation period correlated well with the number of relative light units measured by the HPA. In addition, the relative light unit values of susceptible strains on the first, third and fifth days of incubation were significantly different from those of resistant strains for both isoniazid and rifampin. Our results suggest that after isolation of the organism from clinical specimens, drug-resistant strains of M. tuberculosis are accurately detected by the HPA even after 1 day of incubation with the drug.
摘要
我们开发了一种通过使用杂交保护分析（HPA）来早期检测结核分枝杆菌耐药菌株的新方法。培养期内活细菌的数量与HPA测量的相对光单位数量密切相关。此外，对于异烟肼和利福平，敏感菌株在培养的第一天、第三天和第五天的相对光单位值与耐药菌株的相对光单位值有显著差异。我们的结果表明，从临床标本中分离出该微生物后，即使在与药物孵育1天后，HPA也能准确检测出结核分枝杆菌的耐药菌株。
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2
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