Huang Y T, Johnson R K
Department of Animal Science, University of Nebraska, Lincoln 68583-0908, USA.
J Anim Sci. 1996 Apr;74(4):750-60. doi: 10.2527/1996.744750x.
The objective of this study was to evaluate the effects of selection for increased size of testes on semen and testis characteristics. Boars from a line selected for increased size of testes at 150 d of age (TS, n = 25) and a randomly selected control line (C, n = 18) were used. Semen was collected three times per week for 3 wk (TRT1) then daily for 3 wk (TRT2) beginning when boar age averaged 276 d. It was followed by a 4-wk period of one collection per week and then the TRT1-TRT2 sequence was repeated. After 64 collections, boars were castrated and testes were evaluated for sperm numbers. Volume of semen, percentage of motile sperm cells, and percentage of abnormal cells in the semen did not differ (P > .05) between lines. Concentration of sperm cells in the semen was greater for TS boars at TRT 1 (35.0 million cells/mL, P < .10) and TRT 2 (29.5 million cells/mL, P < .01). Number of sperm cells per ejaculate was greater for TS boars (6.1 and 4.3 billion cells for TRT 1 and TRT 2, respectively; P < .05). Percentage of abnormal cells was less (P < .05) for boars at the younger ages. Concentration of sperm cells was 16 to 18 million cells/mL semen greater (P < .05) at the older ages. Significant differences in the shapes of the response curves with increasing collection number across lines and ages were found. These differences were small and not considered to be important. Line differences in testicular and epididymal sperm numbers and in daily sperm production were consistent with differences in semen traits. Repeatabilities of semen traits were between .16 and .74. Selection for increased size of testes can be used to improve the reproductive capacity of boars used for artificial insemination.
本研究的目的是评估选择增大睾丸体积对公猪精液和睾丸特征的影响。使用了150日龄时经选择增大睾丸体积的品系公猪（TS，n = 25）和随机选择的对照品系公猪（C，n = 18）。从公猪平均年龄276日龄开始，每周采集精液3次，持续3周（TRT1），然后每天采集，持续3周（TRT2）。随后是4周每周采集一次的阶段，然后重复TRT1 - TRT2序列。在采集64次后，对公猪进行阉割，并评估睾丸中的精子数量。两品系之间精液体积、活动精子细胞百分比和精液中异常细胞百分比没有差异（P > .05）。在TRT 1时，TS公猪精液中的精子细胞浓度更高（3500万个细胞/毫升，P < .10），在TRT 2时也更高（2950万个细胞/毫升，P < .01）。TS公猪每次射精的精子细胞数量更多（TRT 1和TRT 2分别为61亿和43亿个细胞；P < .05）。较年轻公猪的异常细胞百分比更低（P < .05）。较年长公猪精液中的精子细胞浓度高1600万至1800万个细胞/毫升（P < .05）。发现不同品系和年龄的公猪随着采集次数增加，反应曲线形状存在显著差异。这些差异很小，不被认为是重要的。睾丸和附睾中的精子数量以及每日精子产量的品系差异与精液性状差异一致。精液性状的重复性在0.16至0.74之间。选择增大睾丸体积可用于提高用于人工授精的公猪的繁殖能力。
