Halpern E P, Bordens R W
Clin Chem. 1979 Jun;25(6):860-2.
We describe the application of the microencapsulated-antibody technique to the radioimmunoassay of digoxin in serum. Droplets of emulsified rabbit antibody are microencapsulated in a semipermeable nylon membrane by an interfacial polymerization technique. The antibody microcapsules are incubated with 125I-labeled digoxin and unlabeled digoxin for 15 min at 37 degrees C, then free and bound digoxin are separated by centrifugation. Subtherapeutic, therapeutic, and toxic concentrations of digoxin in sera can be determined, with use of a standard curve prepared by use of known amounts of digoxin. With this technique we obtained an intra-laboratory correlation coefficient of 0.945 for 100 patients' sera and one of 0.940 for interlaboratory results for 21 sera (10 laboratories) when compared to a routine clinical laboratory radioimmunoassay for digoxin. Icterus, lipemia, hemoglobin, or disproteinemia had no effect on the analytical recovery of digoxin. The standard curve was linear to 6 microgram/L; the sensitivity was 0.25 microgram/L.
我们描述了微囊化抗体技术在血清地高辛放射免疫分析中的应用。通过界面聚合技术，将乳化的兔抗体液滴微囊化于半透性尼龙膜中。抗体微囊与125I标记的地高辛和未标记的地高辛在37℃孵育15分钟，然后通过离心分离游离和结合的地高辛。利用已知量地高辛制备的标准曲线，可测定血清中低于治疗浓度、治疗浓度和中毒浓度的地高辛。与常规临床实验室地高辛放射免疫分析相比，用该技术我们得到100份患者血清的实验室内相关系数为0.945，21份血清（10个实验室）的实验室间结果相关系数为0.940。黄疸、脂血、血红蛋白或蛋白异常对血清地高辛的分析回收率无影响。标准曲线在6μg/L范围内呈线性；灵敏度为0.25μg/L。
