Müller H, Bräuer H, Förster G, Reinhardt M
Klin Wochenschr. 1978 May 15;56(10):493-6. doi: 10.1007/BF01492861.
The results of parallel determinations of digoxin in the sera of non selected patients (n = 104) by enzyme immunoassay (EMIT-EIA) and radioimmunoassay (J-125 labeled RIA) were compared with each other. The determinations revealed considerably different concentrations; the values determined by EIA were statistical lower (for EIA 1.09 +/- 0.99 ng/ml, for RIA 1.34 +/- 1.01 ng/ml, p less than 0.01). In sera with hemolysis and in sera of patients with hyperlipidemia and uremia false-negative results were found by EIA. After elimination of these sera an exact concordation of the values of both the methods was obtained (for EIA 1.12 +/- 1.01 ng/ml, for RIA 1.12 +/- 1.02 ng/ml, r = 0.95).
采用酶免疫分析法（EMIT - EIA）和放射免疫分析法（¹²⁵I标记RIA）对104例未选患者血清中的地高辛进行平行测定，并比较两种方法的结果。测定结果显示浓度差异显著；酶免疫分析法测定值在统计学上较低（酶免疫分析法为1.09±0.99 ng/ml，放射免疫分析法为1.34±1.01 ng/ml，p＜0.01）。在溶血血清、高脂血症患者血清和尿毒症患者血清中，酶免疫分析法出现了假阴性结果。去除这些血清后，两种方法的值完全一致（酶免疫分析法为1.12±1.01 ng/ml，放射免疫分析法为1.12±1.02 ng/ml，r = 0.95）。
