Folkard S G, Hogarth P J, Taylor M J, Bianco A E
Liverpool School of Tropical Medicine, UK.
Parasitology. 1996 Mar;112 ( Pt 3):323-9. doi: 10.1017/s0031182000065847.
Mice inoculated with microfilariae of the filarial nematode Onchocerca lienalis clear their parasites over a period of 3-4 months and are highly resistant to re-infection. We have investigated the comparative roles of the eosinophil, macrophage and neutrophil in effecting this parasite clearance, employing agents specifically to perturb cell function in vivo. Using the anti-IL-5 monoclonal antibody TRFK-5, we show that eosinophils are of primary importance in effecting resistance to re-infection. Ablation of macrophages (with carbon) and neutrophils (with the monoclonal antibody NIMP-R14) had no effect on parasite clearance following re-infection. Neutralization of these 3 cell types during a primary infection showed that while the removal of both eosinophils and macrophages caused a small but significant delay in parasite clearance, the depletion of neutrophils had no effect. This report describes the first direct evidence for eosinophil-mediated killing of microfilariae in vivo, and is consistent with Th-2 cell responses previously described in this model.
用丝状线虫盘尾丝虫的微丝蚴接种的小鼠，在3 - 4个月的时间内清除其体内的寄生虫，并且对再次感染具有高度抗性。我们利用专门在体内干扰细胞功能的试剂，研究了嗜酸性粒细胞、巨噬细胞和中性粒细胞在实现这种寄生虫清除过程中的相对作用。使用抗白细胞介素-5单克隆抗体TRFK-5，我们发现嗜酸性粒细胞在实现对再次感染的抗性方面至关重要。用碳消除巨噬细胞和用单克隆抗体NIMP-R14消除中性粒细胞，对再次感染后的寄生虫清除没有影响。在初次感染期间对这三种细胞类型进行中和作用表明，虽然同时去除嗜酸性粒细胞和巨噬细胞会导致寄生虫清除出现轻微但显著的延迟，但去除中性粒细胞则没有影响。本报告描述了嗜酸性粒细胞在体内介导杀死微丝蚴的首个直接证据，并且与该模型中先前描述的Th-2细胞反应一致。
