Chetty R, Biddolph S C, Kaklamanis L, Cary N, Stewart S, Giatromanolaki A, Gatter K C
University Department of Cellular Science, University of Oxford, Cambridge, UK.
Histopathology. 1996 Mar;28(3):257-60. doi: 10.1046/j.1365-2559.1996.d01-425.x.
An inconsistent association exists between EBV-LMP-1 and bcl-2 protein expression in Reed-Sternberg cells seen in Hodgkin's disease. In fact, many studies have concluded that there is no correlation between EBV-LMP and bcl-2 expression in Hodgkin's disease. We undertook an analysis of post-transplant lymphoproliferative disorders to explore the relationship between EBV-LMP and bcl-2 in Reed-Sternberg-like cells found in this condition, given the strong association between this disorder and EBV. Reed-Sternberg-like cells were found histologically in 11 of 28 cases of renal, heart and heart-lung post-transplant lymphoproliferative disorders. Formalin-fixed, paraffinembedded sections were stained with monoclonal antibodies to EBV-LMP-1 and bcl-2 proteins. Reed-Sternberg-like cells in all 11 cases co-expressed EBV-LMP and bcl-2. A similar relationship was noted with large, mononuclear cells and occasional small lymphoid cells. The staining pattern seen with both antibodies was of similar intensity and both displayed cytoplasmic Golgi accentuation. In the setting of post-transplant lymphoproliferative disorders. Reed-Sternberg-like cells exhibit strong co-expression of EBV-LMP-1 and bcl-2 proteins, supporting a positive correlation between them. This is in contrast to the findings in Hodgkin's disease. The reason for this discrepancy may be due to the iatrogenic immunosuppression and resultant severe EBV infection, together with other cellular events.
在霍奇金病中所见的里德-斯腾伯格细胞中，EBV-LMP-1与bcl-2蛋白表达之间存在不一致的关联。事实上，许多研究得出结论，霍奇金病中EBV-LMP与bcl-2表达之间没有相关性。鉴于移植后淋巴细胞增生性疾病与EBV之间存在密切关联，我们对其进行了分析，以探讨在此疾病中发现的里德-斯腾伯格样细胞中EBV-LMP与bcl-2之间的关系。在28例肾、心脏和心肺移植后淋巴细胞增生性疾病中，有11例在组织学上发现了里德-斯腾伯格样细胞。用抗EBV-LMP-1和bcl-2蛋白的单克隆抗体对福尔马林固定、石蜡包埋的切片进行染色。所有11例中的里德-斯腾伯格样细胞均共表达EBV-LMP和bcl-2。在大的单核细胞和偶尔的小淋巴细胞中也观察到类似的关系。两种抗体的染色模式强度相似，且均显示胞质高尔基体增强。在移植后淋巴细胞增生性疾病的情况下，里德-斯腾伯格样细胞表现出EBV-LMP-1和bcl-2蛋白的强共表达，支持它们之间存在正相关。这与霍奇金病的研究结果形成对比。这种差异的原因可能是医源性免疫抑制以及由此导致的严重EBV感染，以及其他细胞事件。
