Uring-Lambert B, Valette C, Hartmann D, Wolf P, Hauptmann G
Laboratoire de Recherches en Immunologie, Faculté de Médecine, Strasbourg.
Gastroenterol Clin Biol. 1995 Dec;19(12):976-9.
The genes of complement factor B, C2 and C4 are located within the major histocompatibility complex class III region on chromosome 6 in man. These components demonstrate a genetic polymorphism which, when determinated, can be used to define complotypes (association of C2, factor B, C4A and C4B allotypes). On the other hand the liver is the main source of the circulating complement component synthesis. The aim of this study was to analyse the kinetics of several complement component (C3, factor B and C4) concentrations in the plasma and to assess changes in the polymorphic pattern of the complotypes after orthotopic liver transplantation. Nephelometry was used for plasma level measurements and factor B, C2 and C4 typings were performed with high voltage electrophoresis or isofocalisation and immunofixation at intervals before, during and after orthotopic liver transplantation in eleven patients.
Complotypes changes were observed 24 hours after liver transplantation in all patients. A slight decrease in C3, C4, and factor B plasma levels was observed in the first hours after transplantation. A rapid increase in the levels of these components was observed subsequently, with normalization in less than 15 days.
These results demonstrate a rapid synthesis of complement components and the changes in complement polymorphic patterns after liver transplantation.
补体因子B、C2和C4的基因位于人类6号染色体上的主要组织相容性复合体III类区域内。这些成分表现出遗传多态性，确定后可用于定义补体型（C2、因子B、C4A和C4B同种异型的组合）。另一方面，肝脏是循环补体成分合成的主要来源。本研究的目的是分析血浆中几种补体成分（C3、因子B和C4）浓度的动力学，并评估原位肝移植后补体型多态模式的变化。采用散射比浊法测量血浆水平，并在11例患者原位肝移植前、移植期间和移植后的不同时间间隔，通过高压电泳或等聚焦及免疫固定法对因子B、C2和C4进行分型。
所有患者在肝移植后24小时均观察到补体型变化。移植后最初几小时，观察到C3、C4和因子B血浆水平略有下降。随后观察到这些成分的水平迅速升高，不到15天就恢复正常。
这些结果表明肝移植后补体成分迅速合成，补体多态模式发生变化。
