Werner-Washburne M, Braun E L, Crawford M E, Peck V M
Biology Department, University of New Mexico, Albuquerque 87131, USA.
Mol Microbiol. 1996 Mar;19(6):1159-66. doi: 10.1111/j.1365-2958.1996.tb02461.x.
Like other microorganisms, the yeast Saccharomyces cerevisiae responds to starvation by arresting growth and entering stationary phase. Because most microorganisms exist under conditions of nutrient limitation, the ability to tolerate starvation is critical for survival. Molecular analyses have identified changes in transcription, translation, and protein modification in stationary-phase cells. At the level of translation, the pattern of newly synthesized proteins in stationary-phase cells is surprisingly similar to the pattern of proteins synthesized during exponential growth. When limited for different nutrients, yeast strains may not enter stationary phase but opt for pathways such as pseudohyphal growth. If nutrient limitation continues, the end-point is likely to be a stationary-phase cell. Based on the results of recent studies, we propose a model for entry into stationary phase in which G(o) arrest is separable from acquisition of the ability to survive long periods of time without added nutrients.
与其他微生物一样，酿酒酵母通过停止生长并进入稳定期来应对饥饿。由于大多数微生物存在于营养限制的条件下，耐受饥饿的能力对于生存至关重要。分子分析已经确定了稳定期细胞中转录、翻译和蛋白质修饰的变化。在翻译水平上，稳定期细胞中新合成蛋白质的模式与指数生长期合成的蛋白质模式惊人地相似。当受到不同营养物质限制时，酵母菌株可能不会进入稳定期，而是选择假菌丝生长等途径。如果营养限制持续下去，最终结果可能是一个稳定期细胞。基于最近的研究结果，我们提出了一个进入稳定期的模型，其中G(o)停滞与获得在不添加营养物质的情况下长时间存活的能力是可分离的。
