Xia S L, Zhang X, Jing N H
Shanghai Institute of Biochemistry, Academia Sinica.
Shi Yan Sheng Wu Xue Bao. 1995 Dec;28(4):397-407.
PA-1 cell line is derived from human ovary teratocarcinoma. When it grows in 10% fetal calf serum, it can be induced to differentiate by 10(-5) mol/L retinoic acid (RA). Some morphological changes can be observed after RA induction. By immunostaining of cultured cells, we found that the expression and distribution pattern of desmin and some extracellular matrix molecules, such as fibronectin, laminin and tenascin, had been changed after induction, and these changes were associated with the morphological changes. Cell growth study showed that RA treatment had no effects on growth and autocrine activities of PA-1 cells. These results suggest that some PA-1 cells were induced to differentiate along muscle cells pathway by RA.
PA-1细胞系源自人卵巢畸胎癌。当它在10%胎牛血清中生长时，可被10(-5)摩尔/升视黄酸（RA）诱导分化。RA诱导后可观察到一些形态学变化。通过对培养细胞进行免疫染色，我们发现结蛋白以及一些细胞外基质分子，如纤连蛋白、层粘连蛋白和腱生蛋白的表达和分布模式在诱导后发生了改变，且这些变化与形态学变化相关。细胞生长研究表明，RA处理对PA-1细胞的生长和自分泌活性没有影响。这些结果表明，RA可诱导部分PA-1细胞沿肌细胞途径分化。
