Kirsch M, Fuhrmann S, Wiese A, Hofmann H D
Institute of Anatomy I, Albert-Ludwigs-Universität, Freiburg, FRG.
Neuroreport. 1996 Feb 29;7(3):697-700. doi: 10.1097/00001756-199602290-00004.
Effects of ciliary neurotrophic factor (CNTF) on photoreceptor development in dissociated cultures of embryonic chick and newborn rat retina were studied using opsin immunoreactivity to characterize photoreceptor differentiation. In the presence of CNTF, the number of photoreceptors was increased by up to 200% in chick cultures, but was reduced by 82-99% in rat cultures. The EC50 determined for CNTF effects in chick and rat cultures were 0.06 ng ml-1 and 0.02 ng ml-1, respectively. By studying the time course of in vitro development we showed that CNTF transiently stimulated the generation of photoreceptors from opsinnegative precursor cells of chick retina, but completely prevented the same process in rat cultures. These results suggest that CNTF is involved in the regulation of photoreceptor development, but that it can have different actions in the two species, at least in vitro.
利用视蛋白免疫反应性来表征光感受器分化，研究了睫状神经营养因子（CNTF）对鸡胚和新生大鼠视网膜解离培养物中光感受器发育的影响。在存在CNTF的情况下，鸡培养物中的光感受器数量增加了高达200%，但在大鼠培养物中减少了82 - 99%。在鸡和大鼠培养物中确定的CNTF作用的半数有效浓度（EC50）分别为0.06 ng/ml和0.02 ng/ml。通过研究体外发育的时间进程，我们发现CNTF短暂刺激了鸡视网膜视蛋白阴性前体细胞产生光感受器，但在大鼠培养物中完全阻止了相同的过程。这些结果表明CNTF参与了光感受器发育的调节，但至少在体外，它在这两个物种中可能具有不同的作用。
