Voie A H, Spelman F A
Center for Bioengineering, University of Washington, Seattle 98195, USA.
Comput Med Imaging Graph. 1995 Sep-Oct;19(5):377-84. doi: 10.1016/0895-6111(95)00034-8.
This paper describes a methodology for three-dimensional (3D) computer-aided reconstruction of the guinea pig cochlea using orthogonal-plane fluorescence optical sectioning. Specimens are sectioned optically, allowing them to remain intact during observation. Equations to correct the data for specimen translation and rotation are developed and 3D reconstructions of the scala tympani, round window membrane, and cochlear aqueduct are presented. The error associated with the reconstruction is estimated to be < 19 microns.
本文描述了一种使用正交平面荧光光学切片对豚鼠耳蜗进行三维（3D）计算机辅助重建的方法。标本通过光学切片，使其在观察过程中保持完整。推导了用于校正标本平移和旋转数据的方程，并展示了鼓阶、圆窗膜和蜗水管的3D重建。估计重建相关的误差小于19微米。
