Sakuragawa N, Thangavel R, Mizuguchi M, Hirasawa M, Kamo I
Department of Inherited Metabolic Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo 187, Japan.
Neurosci Lett. 1996 May 3;209(1):9-12. doi: 10.1016/0304-3940(96)12599-4.
Human amniotic epithelial (HAE) cells are formed from amnioblasts, separated from the epiblast at about the 8th day after fertilization. We attempted to detect various developmental antigens specific to neural cells by immunocytochemical methods. The cultured HAE cells displayed positive immunoreactivity to RC1, vimentin, A2B5, neurofilament proteins, microtubule-associated protein 2 (MAP2) and MAP2 kinase. In addition, the cells also demonstrated immunoreactivity to glial fibrillary acidic protein, CNPase, myelin basic protein and galactocerebroside. The appearance rate of positive cells was more than 50% in cells positive to RC1, A2B5, vimentin or neuronal markers, and 20-30% to glial cell markers. Double staining showed the heterogeneous appearance of oligodendrocyte lineage cells. These data indicate that HAE cells may have the putative multipotentiality of neurons, astrocytes and oligodendrocytes.
人羊膜上皮（HAE）细胞由成羊膜细胞形成，在受精后约第8天与上胚层分离。我们试图通过免疫细胞化学方法检测神经细胞特有的各种发育抗原。培养的HAE细胞对RC1、波形蛋白、A2B5、神经丝蛋白、微管相关蛋白2（MAP2）和MAP2激酶呈阳性免疫反应。此外，这些细胞对胶质纤维酸性蛋白、2',3'-环核苷酸3'-磷酸二酯酶（CNPase）、髓鞘碱性蛋白和半乳糖脑苷脂也表现出免疫反应。在对RC1、A2B5、波形蛋白或神经元标志物呈阳性的细胞中，阳性细胞的出现率超过50%，对神经胶质细胞标志物呈阳性的细胞中阳性细胞出现率为20%-30%。双重染色显示少突胶质细胞系细胞呈现异质性。这些数据表明，HAE细胞可能具有神经元、星形胶质细胞和少突胶质细胞的假定多能性。
