Bownds S E, Kurzynski T A, Norden M A, Dufek J L, Schell R F
Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison, 53706, USA.
J Clin Microbiol. 1996 Jun;34(6):1386-90. doi: 10.1128/jcm.34.6.1386-1390.1996.
We demonstrated previously that susceptibility testing of Mycobacterium tuberculosis could be accomplished within 24 h after the organisms were incubated with antituberculosis agents by using fluorescein diacetate (FDA) staining and flow cytometry. Continued studies have now shown that assay suspensions containing M. avium, M. fortuitum, M. gordonae, or M. marinum incubated with various concentrations of ciprofloxacin, clarithromycin, erythromycin, kanamycin, rifampin, tobramycin hydrolyzed less FDA than drug-free controls. Suspensions of treated and nontreated mycobacteria could be easily differentiated at 6 and 24 h after the initiation of the susceptibility assays by using FDA staining and flow cytometry. In addition, multiplication of the mycobacteria was not required to discern differences between drug-free suspensions of mycobacteria and those treated with antimycobacterial agents. The flow cytometric assay is simple, reproducible, and rapid.
我们之前证明，通过使用荧光素二乙酸酯（FDA）染色和流式细胞术，结核分枝杆菌与抗结核药物孵育后，药敏试验可在24小时内完成。后续研究现已表明，含有鸟分枝杆菌、偶然分枝杆菌、戈登分枝杆菌或海分枝杆菌的检测悬液，在与不同浓度的环丙沙星、克拉霉素、红霉素、卡那霉素、利福平、妥布霉素孵育后，FDA的水解量比无药对照组少。通过使用FDA染色和流式细胞术，在药敏试验开始后6小时和24小时，可轻松区分经处理和未经处理的分枝杆菌悬液。此外，无需分枝杆菌增殖即可辨别分枝杆菌无药悬液与经抗分枝杆菌药物处理的悬液之间的差异。流式细胞术检测简单、可重复且快速。
