Norden M A, Kurzynski T A, Bownds S E, Callister S M, Schell R F
Wisconsin State Laboratory of Hygiene, Madison 53706, USA.
J Clin Microbiol. 1995 May;33(5):1231-7. doi: 10.1128/jcm.33.5.1231-1237.1995.
The resurgence of tuberculosis has caused considerable effort to be focused on the development of rapid methods for determining the susceptibility of Mycobacterium tuberculosis to antimycobacterial agents. We demonstrated that susceptibility testing of M. tuberculosis can be accomplished rapidly by using flow cytometry. Results of tests were available within 24 h after M. tuberculosis organisms were incubated with ethambutol, isoniazid, rifampin, or streptomycin. The method was based on the ability of viable M. tuberculosis organisms to hydrolyze fluorescein diacetate (FDA) and the detection of fluorescent mycobacteria by flow cytometric analysis. The assay system also did not require multiplication of the mycobacteria. In contrast, M. tuberculosis organisms exposed to antimycobacterial agents hydrolyzed significantly less FDA. The use of flow cytometry and FDA staining shows considerable promise as a rapid method for obtaining susceptibility test results.
结核病的再次流行使得人们投入了大量精力来研发快速测定结核分枝杆菌对抗结核药物敏感性的方法。我们证明，使用流式细胞术可以快速完成结核分枝杆菌的药敏试验。在结核分枝杆菌与乙胺丁醇、异烟肼、利福平或链霉素孵育后24小时内即可获得检测结果。该方法基于活的结核分枝杆菌水解二醋酸荧光素（FDA）的能力以及通过流式细胞术分析检测荧光分枝杆菌。该检测系统也不需要分枝杆菌繁殖。相比之下，暴露于抗结核药物的结核分枝杆菌水解的FDA明显较少。流式细胞术和FDA染色的应用作为一种获取药敏试验结果的快速方法显示出了巨大的前景。
