通过流式细胞术对白色念珠菌进行快速药敏试验。
Rapid susceptibility testing of Candida albicans by flow cytometry.
作者信息
Kirk S M, Callister S M, Lim L C, Schell R F
机构信息
Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison 53706, USA.
出版信息
J Clin Microbiol. 1997 Feb;35(2):358-63. doi: 10.1128/jcm.35.2.358-363.1997.
Abstract
The emerging magnitude of human fungal infections has renewed interest in developing rapid and standardized methods for susceptibility testing. We demonstrated that susceptibility testing of Candida albicans can be accomplished rapidly by using flow cytometry. Test results were available within 8 to 24 h after C. albicans isolates were incubated with amphotericin B, itraconazole, and flucytosine. This is an improvement of 24 to 60 h in the time to availability of susceptibility test results compared to the time to availability of National Committee for Clinical Laboratory Standards-recommended broth macrodilution test results. In addition, the flow cytometric endpoints, mean channel fluorescence, and number of fluorescence-labeled C. albicans cells were easy to interpret for greater sensitivity and reliability. Flow cytometry provides a more accurate means of obtaining antifungal susceptibility test results.
摘要
人类真菌感染日益严重，这重新激发了人们对开发快速且标准化的药敏试验方法的兴趣。我们证明，通过使用流式细胞术可以快速完成白色念珠菌的药敏试验。将白色念珠菌分离株与两性霉素B、伊曲康唑和氟胞嘧啶孵育后，8至24小时内即可获得试验结果。与美国国家临床实验室标准委员会推荐的肉汤稀释法药敏试验结果报告时间相比，这将药敏试验结果报告时间缩短了24至60小时。此外，流式细胞术的终点指标、平均通道荧光强度和荧光标记的白色念珠菌细胞数量易于解读，具有更高的灵敏度和可靠性。流式细胞术为获得抗真菌药敏试验结果提供了一种更准确的方法。
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