Heifets L B, Iseman M D, Cook J L, Lindholm-Levy P J, Drupa I
Antimicrob Agents Chemother. 1985 Jan;27(1):11-5. doi: 10.1128/AAC.27.1.11.
Among eight cephalosporins and cephamycins tested in preliminary in vitro screening against Mycobacterium tuberculosis, the most promising for further study was found to be ceforanide, followed by ceftizoxime, cephapirin, and cefotaxime. Moxalactam, cefoxitin, cefamandole, and cephalothin were found to be not active enough against M. tuberculosis to be considered for further in vitro studies. The antibacterial activity of various ceforanide concentrations was investigated by three methods: (i) the dynamics of radiometric readings (growth index) in 7H12 broth; (ii) the number of CFU in the same medium; and (iii) the proportion method on 7H11 agar plates. There was a good correlation among the results obtained with these methods. The MIC for most strains ranged from 6.0 to 25.0 micrograms/ml. The BACTEC radiometric method is a reliable, rapid, and convenient method for preliminary screening and determination of the level of antibacterial activity of drugs not commonly used against M. tuberculosis.
在针对结核分枝杆菌进行的初步体外筛选试验中测试的8种头孢菌素和头霉素中，发现最有进一步研究前景的是头孢尼西，其次是头孢唑肟、头孢匹林和头孢噻肟。发现拉氧头孢、头孢西丁、头孢孟多和头孢噻吩对结核分枝杆菌的活性不足，不值得考虑进一步进行体外研究。通过三种方法研究了不同浓度头孢尼西的抗菌活性：（i）7H12肉汤中放射性读数（生长指数）的动态变化；（ii）同一培养基中的菌落形成单位数量；（iii）7H11琼脂平板上的比例法。这些方法得到的结果之间具有良好的相关性。大多数菌株的最低抑菌浓度范围为6.0至25.0微克/毫升。BACTEC放射性测定法是一种可靠、快速且方便的方法，可用于对不常用于抗结核分枝杆菌的药物进行初步筛选和抗菌活性水平的测定。
