Dijkshoorn L, Aucken H, Gerner-Smidt P, Janssen P, Kaufmann M E, Garaizar J, Ursing J, Pitt T L
Department of Medical Microbiology, Leiden University Hospital, The Netherlands.
J Clin Microbiol. 1996 Jun;34(6):1519-25. doi: 10.1128/jcm.34.6.1519-1525.1996.
Thirty-one Acinetobacter baumannii strains, comprising 14 strains from 14 outbreaks in different northwestern European cities and 17 sporadic strains, were compared by investigating various properties of the strains including biotype, antibiogram, cell envelope protein electrophoretic profile, ribotype pattern, and the band pattern generated by a novel genomic fingerprinting method, named AFLP, which is based on the selective amplification of restriction fragments. Results showed that 12 strains from unrelated outbreaks were linked together in two clusters according to their similarities by these typing methods, whereas sporadic strains were more heterogeneous. Outbreak strains appeared to be markedly more resistant to antibiotics than nonoutbreak strains. The uniformity of typing characters in two sets of outbreak strains suggests that strains in each cluster have a common clonal origin.
对31株鲍曼不动杆菌进行了比较，其中包括来自欧洲西北部不同城市14次暴发的14株菌株以及17株散发病例菌株。通过研究这些菌株的各种特性，包括生物型、抗菌谱、细胞包膜蛋白电泳图谱、核糖体分型模式以及一种名为AFLP的新型基因组指纹图谱方法（基于限制性片段的选择性扩增）所产生的条带模式，来进行比较。结果显示，根据这些分型方法，来自不相关暴发的12株菌株按其相似性被归为两个簇，而散发病例菌株则更为异质。暴发菌株似乎比非暴发菌株对抗生素的耐药性明显更强。两组暴发菌株分型特征的一致性表明，每个簇中的菌株具有共同的克隆起源。
