通过基因型和表型方法对鲍曼不动杆菌暴发株和非暴发株进行比较。
Comparison of outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods.
作者信息
Dijkshoorn L, Aucken H, Gerner-Smidt P, Janssen P, Kaufmann M E, Garaizar J, Ursing J, Pitt T L
机构信息
Department of Medical Microbiology, Leiden University Hospital, The Netherlands.
出版信息
J Clin Microbiol. 1996 Jun;34(6):1519-25. doi: 10.1128/jcm.34.6.1519-1525.1996.
Abstract
Thirty-one Acinetobacter baumannii strains, comprising 14 strains from 14 outbreaks in different northwestern European cities and 17 sporadic strains, were compared by investigating various properties of the strains including biotype, antibiogram, cell envelope protein electrophoretic profile, ribotype pattern, and the band pattern generated by a novel genomic fingerprinting method, named AFLP, which is based on the selective amplification of restriction fragments. Results showed that 12 strains from unrelated outbreaks were linked together in two clusters according to their similarities by these typing methods, whereas sporadic strains were more heterogeneous. Outbreak strains appeared to be markedly more resistant to antibiotics than nonoutbreak strains. The uniformity of typing characters in two sets of outbreak strains suggests that strains in each cluster have a common clonal origin.
摘要
对31株鲍曼不动杆菌进行了比较，其中包括来自欧洲西北部不同城市14次暴发的14株菌株以及17株散发病例菌株。通过研究这些菌株的各种特性，包括生物型、抗菌谱、细胞包膜蛋白电泳图谱、核糖体分型模式以及一种名为AFLP的新型基因组指纹图谱方法（基于限制性片段的选择性扩增）所产生的条带模式，来进行比较。结果显示，根据这些分型方法，来自不相关暴发的12株菌株按其相似性被归为两个簇，而散发病例菌株则更为异质。暴发菌株似乎比非暴发菌株对抗生素的耐药性明显更强。两组暴发菌株分型特征的一致性表明，每个簇中的菌株具有共同的克隆起源。
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本文引用的文献
1
Correlation of typing methods for Acinetobacter isolates from hospital outbreaks.医院暴发中不动杆菌分离株分型方法的相关性
J Clin Microbiol. 1993 Mar;31(3):702-5. doi: 10.1128/jcm.31.3.702-705.1993.
2
Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates.基于全细胞重复元件序列的聚合酶链反应可快速评估细菌分离株的克隆关系。
J Clin Microbiol. 1993 Jul;31(7):1927-31. doi: 10.1128/jcm.31.7.1927-1931.1993.
3
Endemic acinetobacter in intensive care units: epidemiology and clinical impact.重症监护病房中的地方性不动杆菌：流行病学及临床影响
J Clin Pathol. 1993 Jun;46(6):533-6. doi: 10.1136/jcp.46.6.533.
4
5
Numerical classification and identification of Acinetobacter genomic species.不动杆菌基因组种的数值分类与鉴定
J Appl Bacteriol. 1993 Sep;75(3):259-68. doi: 10.1111/j.1365-2672.1993.tb02775.x.
6
Plasmid DNA profiles of Acinetobacter baumannii: clinical application in a complex endemic setting.鲍曼不动杆菌的质粒DNA图谱：在复杂地方病环境中的临床应用
Infect Control Hosp Epidemiol. 1994 Aug;15(8):520-8. doi: 10.1086/646970.
7
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J Hosp Infect. 1993 Sep;25(1):15-32. doi: 10.1016/0195-6701(93)90005-k.
8
Phenotypic characterisation of Acinetobacter strains of 13 DNA-DNA hybridisation groups by means of the biolog system.利用Biolog系统对13个DNA-DNA杂交组不动杆菌菌株进行表型特征分析。
J Med Microbiol. 1995 Feb;42(2):113-9. doi: 10.1099/00222615-42-2-113.
9
Clinical and epidemiological investigations of Acinetobacter genomospecies 3 in a neonatal intensive care unit.新生儿重症监护病房中不动杆菌基因组种3的临床和流行病学调查
J Clin Microbiol. 1995 Jun;33(6):1567-72. doi: 10.1128/jcm.33.6.1567-1572.1995.
10
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J Clin Microbiol. 1995 Jun;33(6):1537-47. doi: 10.1128/jcm.33.6.1537-1547.1995.
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