Hsu H Y, Ho Y H, Lin C C
Department of Radiotherapy and School of Pharmacy, Kaohsiung Medical College, Taiwan, R.O.C.
J Ethnopharmacol. 1996 Jun;52(2):113-7. doi: 10.1016/0378-8741(96)01400-6.
The ability of Si-Wu-Tang to eliminate bone marrow damage after radiation was assessed using both a clonogenic assay (survival of colony forming units spleen--CFUs) of stem cell survival and a hematopoietic functional assay including the changes of hemograms and hematocrit. Cell survival curves and dose-response curves for radiation alone and Si-Wu-Tang administration with radiation were constructed over the dose range of 1 to 9 Gy. Si-Wu-Tang was given 7 days before irradiation at a consecutive fractionated dose of 20 mg/20 g body weight. The radioresistance of stem cells treated by Si-Wu-Tang was higher than that of those treated by irradiation alone. However, the anti-radiation effect of leukocytes was not as significant as that of erythrocytes, thrombocytes and hematocrit. These data suggest that the anti-radiation effect by Si-Wu-Tang is dependent on dose and will be less after 4 Gy irradiation. Hemograms with a short turnover period, such as leukocytes, may be less affected after Si-Wu-Tang administration.
采用干细胞存活的克隆形成试验（脾集落形成单位——CFUs的存活）和包括血细胞计数及血细胞比容变化的造血功能试验，评估四物汤对辐射后骨髓损伤的消除能力。在1至9 Gy的剂量范围内，构建了单独辐射以及辐射加四物汤给药的细胞存活曲线和剂量反应曲线。在照射前7天，以20 mg/20 g体重的连续分次剂量给予四物汤。四物汤处理的干细胞的辐射抗性高于单独照射处理的干细胞。然而，白细胞的抗辐射作用不如红细胞、血小板和血细胞比容显著。这些数据表明，四物汤的抗辐射作用取决于剂量，且在4 Gy照射后作用会减弱。周转期短的血细胞，如白细胞，在给予四物汤后可能受影响较小。
