Duménigo B E, Espino A M, Finlay C M
Instituto Medicina Tropical Pedro Kourt, La Habana, Cuba.
Res Vet Sci. 1996 May;60(3):278-9. doi: 10.1016/s0034-5288(96)90055-7.
A monoclonal antibody-based sandwich immunoassay (mAb sandwich ELISA) was developed for the detection of Fasciola hepatica antigen in the faeces of cattle. The assay was applied to samples from 100 cattle infected with F hepatica, 56 animals with parasitologically proven infections of other parasites and 100 uninfected animals. F hepatica antigen was detected in all the faecal samples from animals with fasciolosis, but none of the samples from the uninfected animals or from those with other parasitic infections had significant levels of F hepatica antigens. The results indicate that the mAb sandwich ELISA is a rapid, simple and useful method for the diagnosis of active F hepatica infection in cattle.
开发了一种基于单克隆抗体的夹心免疫测定法（单克隆抗体夹心酶联免疫吸附测定法），用于检测牛粪便中的肝片吸虫抗原。该测定法应用于来自100头感染肝片吸虫的牛、56头经寄生虫学证实感染其他寄生虫的动物以及100头未感染动物的样本。在所有患有肝片吸虫病动物的粪便样本中均检测到肝片吸虫抗原，但未感染动物或患有其他寄生虫感染动物的样本中均未检测到显著水平的肝片吸虫抗原。结果表明，单克隆抗体夹心酶联免疫吸附测定法是诊断牛活动性肝片吸虫感染的一种快速、简便且有用的方法。
