评价Etest法对鸟分枝杆菌进行克拉霉素快速药敏试验的效果。

Evaluation of the Etest for rapid susceptibility testing of Mycobacterium avium to clarithromycin.

作者信息

Lebrun L, Onody C, Vincent V, Nordmann P

机构信息

Service de Bactériologie-Virologie, Hôpital Antoine Béclère, Clamart, France.

出版信息

J Antimicrob Chemother. 1996 May;37(5):999-1003. doi: 10.1093/jac/37.5.999.

DOI:10.1093/jac/37.5.999

PMID:8737150

Abstract

MICs of clarithromycin were determined by the Etest method for thirty clinical strains of Mycobacterium avium complex (MAC) and compared with MICs results as determined by the reference agar dilution method. Agreement (within +/- 1 log2 dilution) between the Etest and the reference method was 70% for susceptible strains and 100% for resistant strains. No major errors resulting in misclassification in susceptibility or resistance categories were detected for the Etest MIC method. It is suggested that the Etest is easy to perform and is an accurate method for determining susceptibility of MAC strains to clarithromycin.

摘要

采用Etest法测定了30株鸟分枝杆菌复合群（MAC）临床菌株对克拉霉素的最低抑菌浓度（MIC），并与参考琼脂稀释法测定的MIC结果进行比较。对于敏感菌株，Etest法与参考方法的一致性（在±1个log2稀释度范围内）为70%，对于耐药菌株为100%。未检测到Etest MIC法导致药敏或耐药类别误判的重大误差。提示Etest法操作简便，是测定MAC菌株对克拉霉素药敏性的准确方法。