Quantitation of urinary immunogloublins by a double antibody technique.

A double antibody method for quantitation of urinary immunoglobulins that is sensitive enough to avoid the need for urine concentrations has been developed. The first antibody is goat antiserum monospecific for the heavy chains of IgA, IgG, and IgM. The second, and precipitating, antibody is developed in rabbits made tolerant to human IgG. Normal values for male and female children and adults are presented. Elevated levels of urinary IgA and IgG were found after urinary tract infection.