Wang Y L, Tang G Z, Ding J H, Li D X
Department of Pharmacology, Nanjing Medical University, China.
Zhongguo Yao Li Xue Bao. 1996 Jan;17(1):38-40.
To study the effect of nitrendipine (Nit) on the capacity of calcium binding of erythrocyte membrane and total intraerythrocyte calcium content in spontaneous hypertensive rats (SHR).
Systolic blood pressure (SBP) of the conscious rats was monitored by tailcuff method with a BP and HR recorder for MRS-III rat. Erythrocyte membrane was prepared according to modified Bing's method. Calcium binding of membrane and total intraerythrocyte calcium content was determined by an automatic absorption spectrophotometer. The membrane protein was determined with a colorimetric method.
Nit (ig 10 mg.kg-1 qd x 20 d) induced a significant reduction in total intraerythrocyte calcium content (169 +/- 18 vs 87 +/- 14 mumol/L cell, P < 0.01) accompanied by a marked fall of SBP (27.1 +/- 2.5 vs 16.7 +/- 1.0 kPa, P < 0.01) but exerted no influence on the capacity of calcium binding of erythrocyte membrane under incubation in CaCl2(0) (basal calcium binding) or 40 mmol.L-1 (maximal calcium binding) (21.9 +/- 2.3 vs 22.7 +/- 2.1 and 55 +/- 14 vs 53 +/- 23 mumol/g protein, respectively, P > 0.05).
The antihypertensive effect of Nit is related to the reduction of intracellular calcium and possibly have no direct relation to the capacity of calcium binding of cell membrane.
研究尼群地平（Nit）对自发性高血压大鼠（SHR）红细胞膜钙结合能力及红细胞内总钙含量的影响。
采用MRS-III型大鼠血压和心率记录仪，通过尾套法监测清醒大鼠的收缩压（SBP）。按照改良的Bing法制备红细胞膜。用自动吸收分光光度计测定膜钙结合能力及红细胞内总钙含量。用比色法测定膜蛋白。
尼群地平（灌胃10mg·kg-1，每日1次，共20天）使红细胞内总钙含量显著降低（169±18 vs 87±14μmol/L细胞，P<0.01），同时收缩压明显下降（27.1±2.5 vs 16.7±1.0kPa，P<0.01），但对在CaCl2（0）（基础钙结合）或40mmol·L-1（最大钙结合）孵育条件下的红细胞膜钙结合能力无影响（分别为21.9±2.3 vs 22.7±2.1和55±14 vs 53±23μmol/g蛋白，P>0.05）。
尼群地平的降压作用与细胞内钙减少有关，可能与细胞膜钙结合能力无直接关系。
