Salojin K V, Le Tonquèze M, Nassovov E L, Blouch M T, Baranov A A, Saraux A, Guillevin L, Fiessinger J N, Piette J C, Youinou P
Laboratory of Immunology, Brest University Medical School, France.
Clin Exp Rheumatol. 1996 Mar-Apr;14(2):163-9.
To determine the prevalence of anti-endothelial cell antibodies (AECA) in various forms of vasculitis and to evaluate their relationships with markers of endothelial cell (EC) injury such as thrombomodulin (TM), von Willebrand factor antigen (vWf) and protein S.
A total of 167 disease-associated sera, from 79 patients with large- or medium-sized (group I) and 88 with small-sized vessel vasculitis (group II), were examined for the presence of AECA using a cellular enzyme-linked immunosorbent assay (ELISA). These were evaluated before and after incubation with epithelial cells. EC plus epithelial cell (eC) extracts were fractionated and blotted with selected sera, and EC plus mononuclear cell extracts were dotted and blotted with lupus sera. Soluble TM, vWf and protein S levels were measured by ELISA.
The binding of antibodies to eC was significant in group II sera (p < 0.01) but not in group I sera, so that the remaining EC-specific activity was significantly higher (p < 0.001) in the latter group than in the former. Eight antigenic specificities appeared to be specific for EC, whereas three were shared with eC. Despite absorption, the sera remained as reactive with EC and MNC as before. Taking the patient group as a whole, the levels of serum TM correlated with the titers of IgG, IgM and IgA AECA.
EC-specific activity is more often encountered in group I than in group II patients. At present, the explanation for the distinct AECA specificities in these disease associated sera is not clear.
确定各种类型血管炎中抗内皮细胞抗体（AECA）的患病率，并评估其与内皮细胞（EC）损伤标志物如血栓调节蛋白（TM）、血管性血友病因子抗原（vWf）和蛋白S的关系。
使用细胞酶联免疫吸附测定（ELISA）检测了167份与疾病相关的血清，这些血清来自79例大中型血管炎患者（I组）和88例小血管血管炎患者（II组）。在与上皮细胞孵育前后对这些血清进行评估。将EC加上皮细胞（eC）提取物进行分级分离并用选定的血清进行印迹，将EC加单核细胞提取物进行斑点印迹并用狼疮血清进行印迹。通过ELISA测量可溶性TM、vWf和蛋白S水平。
II组血清中抗体与eC的结合具有显著性（p < 0.01），而I组血清中则无显著性，因此I组中剩余的EC特异性活性显著高于II组（p < 0.001）。八种抗原特异性似乎对EC具有特异性，而三种与eC共有。尽管进行了吸收处理，但血清与EC和MNC的反应性仍与之前相同。将患者组作为一个整体，血清TM水平与IgG、IgM和IgA AECA的滴度相关。
I组患者比II组患者更常出现EC特异性活性。目前，这些与疾病相关血清中AECA特异性不同的原因尚不清楚。
