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The isolation and mapping of 19 tetranucleotide microsatellite markers in the chicken.

作者信息

McConnell S K, Dawson D A, Wardle A, Burke T

机构信息

Department of Biology, University of Leicester, UK.

出版信息

Anim Genet. 1999 Jun;30(3):183-9. doi: 10.1046/j.1365-2052.1999.00454.x.

DOI:10.1046/j.1365-2052.1999.00454.x
PMID:10442979
Abstract

Microsatellites consisting of tetranucleotide repeats are more easily, and consequently efficiently, scored than loci consisting of dinucleotides. However, they are much less frequent in the genome. A hybridisation selection protocol was therefore employed to generate a chicken genomic library enriched for inserts containing the tetranucleotide repeat motif (TTTC)n. Forty-five new microsatellite sequences were isolated that mainly consisted of perfect repeats of the tetranucleotide (TTTC) motif. Nineteen markers were mapped in one or both of the East Lansing and Compton international chicken reference populations.

摘要

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