Cerretani D, Micheli L, Fiaschi A I, Giorgi G
Institute of Pharmacology, University of Siena, Italy.
J Chromatogr B Biomed Appl. 1996 Apr 12;678(2):365-8. doi: 10.1016/0378-4347(95)00518-8.
A simple high-performance liquid chromatographic (HPLC) method for the determination of flufenamic acid in rat plasma is described. After liquid-liquid extraction, the drug is separated by HPLC on a 5-microns octadecylsilica column (Nucleosil C18) with ultraviolet detection at 280 nm. Linear calibration graphs for flufenamic acid were constructed from 0.5 to 15 micrograms/ml. The method has been applied to a pharmacokinetic study in animals.
描述了一种用于测定大鼠血浆中氟芬那酸的简单高效液相色谱（HPLC）方法。液液萃取后，药物在5微米十八烷基硅胶柱（Nucleosil C18）上通过HPLC分离，并在280nm处进行紫外检测。氟芬那酸的线性校准曲线在0.5至15微克/毫升范围内构建。该方法已应用于动物的药代动力学研究。
