Stringer J L
Department of Pharmacology, Baylor College of Medicine, Houston TX 77030, USA.
Brain Res. 1996 Apr 22;717(1-2):147-53. doi: 10.1016/0006-8993(96)00059-5.
Reactive gliosis is a response of astrocytes to a variety of insults that is characterized by hypertrophy of the cell bodies and processes and an increase in the expression of glial fibrillary acidic protein (GFAP). The signal that regulates the transition to the reactive state and the role of vimentin in reactive gliosis are unknown. The experiments here used a model of repeated seizures in the hippocampal-parahippocampal circuits to determine the extent and time course of reactive gliosis, including the appearance of vimentin, in response to seizures. Reactive gliosis, detected by immunohistochemistry for the presence of GFAP and vimentin, was present 2-7 days after the repeated seizures. At least 9 seizures, or at least 250 s of seizure discharge, were needed to induce reactive gliosis. After seizures, cells staining for vimentin were found in the dentate gyrus molecular layer and hilar region, as well as in the molecular layer of CA1. Fewer cells were stained in the CA3 region. These experiments demonstrate that repeated discrete seizures of the hippocampal-parahippocampal circuits can cause reactive gliosis and localized induction of a glial protein (vimentin) that is not normally expressed in the adult brain.
反应性胶质增生是星形胶质细胞对多种损伤的一种反应，其特征为细胞体和突起肥大以及胶质纤维酸性蛋白（GFAP）表达增加。调节向反应性状态转变的信号以及波形蛋白在反应性胶质增生中的作用尚不清楚。此处的实验使用海马-海马旁回环路反复癫痫发作模型来确定反应性胶质增生的程度和时间进程，包括癫痫发作后波形蛋白的出现情况。通过免疫组织化学检测GFAP和波形蛋白的存在来检测反应性胶质增生，其在反复癫痫发作后2至7天出现。诱导反应性胶质增生至少需要9次癫痫发作或至少250秒的癫痫放电。癫痫发作后，在齿状回分子层和门区以及CA1分子层中发现了波形蛋白染色的细胞。CA3区染色的细胞较少。这些实验表明，海马-海马旁回环路反复离散性癫痫发作可导致反应性胶质增生以及在成年大脑中通常不表达的一种胶质蛋白（波形蛋白）的局部诱导。
