Adams B, Von Ling E, Vaccarella L, Ivy G O, Fahnestock M, Racine R J
Department of Psychology, McMaster University, Hamilton, Ontario, Canada.
Brain Res. 1998 Sep 7;804(2):331-6. doi: 10.1016/s0006-8993(98)00605-2.
Recurrent seizure activity induced during kindling has been reported to cause an increase in the hilar area of the dentate gyrus of the hippocampus. To date, very little is known about the mechanism of this increase. This study investigated the time course for kindling-induced changes in the hilar area of the dentate gyrus at seven days, one month, and two months post-kindling. Hilar area of the dentate gyrus was significantly increased by approximately 46% at seven days and remained elevated at one month, but declined back to control levels by two months. Glial fibrillary acidic protein (GFAP) immunostaining was also evaluated at the same time points to determine whether kindling-induced changes in the hilar area of the dentate gyrus are related to kindling-induced glial cell changes. Increases in hilar GFAP immunostaining by approximately 57% were observed at seven days and at one month post-kindling, but not at two months post-kindling. These findings indicate that kindling-induced changes in the hilar area of the dentate gyrus and kindling-induced glial cell changes follow a similar time course, and that kindling-induced glial cell changes may mediate the observed changes in the hilar area of the dentate gyrus.
据报道，点燃过程中诱发的反复癫痫发作活动会导致海马齿状回门区增大。迄今为止，对于这种增大的机制知之甚少。本研究调查了点燃后7天、1个月和2个月时，点燃诱导的齿状回门区变化的时间进程。齿状回门区在7天时显著增大了约46%，并在1个月时保持升高，但在2个月时回落至对照水平。同时还在相同时间点评估了胶质纤维酸性蛋白（GFAP）免疫染色，以确定点燃诱导的齿状回门区变化是否与点燃诱导的神经胶质细胞变化有关。在点燃后7天和1个月时，观察到门区GFAP免疫染色增加了约57%，但在点燃后2个月时未观察到增加。这些发现表明，点燃诱导的齿状回门区变化和点燃诱导的神经胶质细胞变化遵循相似的时间进程，并且点燃诱导的神经胶质细胞变化可能介导了观察到的齿状回门区变化。
