Wilson C B, Leopard J, Cheresh D A, Nakamura R M
Department of Immunology, Scripps Research Institute, La Jolla, California 92037-1093, USA.
World J Urol. 1996;14 Suppl 1:S30-7. doi: 10.1007/BF00182062.
We performed an immunohistochemistry study of the normal human bladder so as to understand the interactions of extracellular matrix (ECM) components and the integrins of cell adhesion that accommodate the volume changes and maintain an impermeable barrier to reabsorption of urine in the bladder. The normal human urothelial cell and/or its plasma membrane contained integrins alpha 3, alpha V, beta 1, and beta 4 but did not contain integrin beta 3. The urothelial basement membrane (UBM) contained collagen type IV and laminin. Fibronectin and integrins alpha 3 and beta 4 were found in or near the UBM area, with types I and III collagen and tenascin abutting the area. The patterns of collagen, laminin, tenascin, vitronectin, fobronectin, and the alpha 3, alpha V, beta 1, and beta 3 integrins in the lamina propria, vessels, nerves, and smooth-muscle layers are described. These findings detail the normal anatomical ECM/integrin relationship that provides the cellular basis for bladder-wall relationships responsible for its impermeable state and other functions.
我们对正常人体膀胱进行了免疫组织化学研究，以了解细胞外基质（ECM）成分与细胞黏附整合素之间的相互作用，这些整合素可适应膀胱的容积变化，并维持对尿液重吸收的不透水屏障。正常人体尿路上皮细胞和/或其质膜含有整合素α3、αV、β1和β4，但不含有整合素β3。尿路上皮基底膜（UBM）含有IV型胶原蛋白和层粘连蛋白。在UBM区域内或附近发现了纤连蛋白以及整合素α3和β4，I型和III型胶原蛋白以及腱生蛋白邻接该区域。本文描述了固有层、血管、神经和平滑肌层中胶原蛋白、层粘连蛋白、腱生蛋白、玻连蛋白、纤连蛋白以及α3、αV、β1和β3整合素的分布模式。这些发现详细阐述了正常的解剖学ECM/整合素关系，为膀胱壁处于不透水状态及其他功能的细胞基础提供了依据。
