Tiitta O, Wahlström T, Virtanen I, Gould V E
Department of Anatomy, University of Helsinki, Finland.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1993;63(5):283-7. doi: 10.1007/BF02899274.
Tenascin (Tn) is an extracellular matrix (ECM) glycoprotein strongly and widely expressed during embryogenesis. Tn is decreased in normal adult tissues but is reexpressed in numerous inflammatory, reparative and neoplastic processes. We immunostained samples of fetal and normal adult bladders and samples of bladder tissue from patients with chronic cystitis, detrusor hypertrophy, malakoplakia and transitional cell carcinomas (TCC) of all grades, with a monoclonal antibody (mAb) to Tn 143DB7. Sections of flat in situ carcinomas were also studied. In fetal bladders, strong and ragged Tn reactions were noted at the epithelial-stromal interface; in normal adult bladders, the reaction was delicate and less extensive. In chronic cystitis, Tn reactivity was enhanced particularly around prominent capillary blood vessels. In flat in situ carcinomas, Tn staining was stronger and more extensive than in normal mucosa but was often less extensive than in some examples of cystitis. In TCC I and II, Tn immunoreactivity was strong and predominated in the pericapillary stroma of the papillae; in infiltrating TCC II, comparatively limited Tn staining was noted. In deeply infiltrating grade III TCC with abundant stroma, Tn reaction was invariably strong and extensive, particularly around advancing tumor nests. The strongest Tn reactions were noted in invasive, high-grade TCC with abundant stroma. We conclude that in inflammatory-reactive processes, and in in situ carcinomas as well as in TCC, the extent and intensity of the Tn reaction correlates with the severity of the inflammatory infiltrate and with the extent of the stromal remodelling.
腱生蛋白（Tn）是一种细胞外基质（ECM）糖蛋白，在胚胎发生过程中强烈且广泛表达。Tn在正常成体组织中减少，但在许多炎症、修复和肿瘤形成过程中重新表达。我们用抗Tn 143DB7单克隆抗体（mAb）对胎儿和正常成人膀胱样本以及慢性膀胱炎、逼尿肌肥大、软斑病和各级移行细胞癌（TCC）患者的膀胱组织样本进行免疫染色。还研究了扁平原位癌切片。在胎儿膀胱中，在上皮-基质界面处观察到强烈且不规则的Tn反应；在正常成人膀胱中，反应微弱且范围较小。在慢性膀胱炎中，Tn反应性增强，尤其是在突出的毛细血管周围。在扁平原位癌中，Tn染色比正常黏膜更强且更广泛，但通常比某些膀胱炎病例的范围小。在TCC I和II中，Tn免疫反应性强，在乳头的毛细血管周围基质中占主导；在浸润性TCC II中，观察到相对有限的Tn染色。在具有丰富基质的深度浸润性III级TCC中，Tn反应始终强烈且广泛，尤其是在进展中的肿瘤巢周围。在具有丰富基质的浸润性高级别TCC中观察到最强的Tn反应。我们得出结论，在炎症反应过程、原位癌以及TCC中，Tn反应的程度和强度与炎症浸润的严重程度以及基质重塑的程度相关。
