Hidaka S, Okamoto Y, Abe K, Miyazaki K, Suekawa M, Liu S Y
Department of Oral Biochemistry, Fukuoka Dental College, Japan.
Am J Chin Med. 1996;24(1):65-75. doi: 10.1142/S0192415X96000098.
The effects of 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) and two Chinese traditional (Kampo) medicines, Dai-saiko-to and Saiko-ka-ryukotsu-borei-to on spontaneous soft tissue (heart and tongue) mineralization in DBA/2NCrj mice were studied. These agents were given orally for 4 weeks to DBA/2NCrj mice. After 2 weeks of administration in the heart, 0.006 and 0.03% (w/v) HEBP decreased calcium content by 90 and 30%, respectively, while 0.27 and 2.7 mg/ml Dai-saiko-to reduced calcium content by 30 and 45%, respectively. Saiko-ka-ryukotsu-borei-to (0.27 mg/ml) reduced both calcium and phosphorus content by 50 and 35%, respectively. However, their inhibitory effects on the heart were not observed after 4 weeks of administration. The compounds delayed the onset of increases of bulk calcium and phosphorus content. In the tongue, at 4 weeks, 0.006 and 0.03% (w/v) HEBP reduced calcium content by 30 and 45%, respectively, while two Kampo medicines (at both concentrations used) significantly reduced the content of calcium (by 27-79%) and phosphorus (by 24-32%). These results strongly suggest that two Kampo medicines as well as HEBP may be useful in preventing and curing soft tissue calcification.
研究了1-羟基亚乙基-1,1-二膦酸酯（HEBP）以及两种汉方（日本 Kampo）药——柴芍六君子汤和柴胡龙骨牡蛎汤对 DBA/2NCrj 小鼠自发性软组织（心脏和舌头）矿化的影响。将这些药剂口服给予 DBA/2NCrj 小鼠，持续4周。在给药2周后，心脏中，0.006%和0.03%（w/v）的 HEBP 分别使钙含量降低了90%和30%，而0.27 mg/ml 和2.7 mg/ml 的柴芍六君子汤分别使钙含量降低了30%和45%。柴胡龙骨牡蛎汤（0.27 mg/ml）使钙和磷含量分别降低了50%和35%。然而，给药4周后未观察到它们对心脏的抑制作用。这些化合物延迟了总钙和磷含量增加的起始时间。在舌头中，4周时，0.006%和0.03%（w/v）的 HEBP 分别使钙含量降低了30%和45%，而两种汉方药物（在所用的两种浓度下）均显著降低了钙（降低27%-79%）和磷（降低24%-32%）的含量。这些结果有力地表明，两种汉方药物以及 HEBP 可能对预防和治疗软组织钙化有用。
