Spike C A, Bumstead N, Crittenden L B, Lamont S J
Department of Animal Science, Iowa State University, Ames 50011-3150, USA.
J Hered. 1996 Jan-Feb;87(1):6-9. doi: 10.1093/oxfordjournals.jhered.a022954.
Chicken cDNA probes were used to map 10 loci by restriction fragment length polymorphism (RFLP) analysis in progeny of the Jungle Fowl x White Leghorn chicken backcross gene mapping reference population. The cDNA probes were from a subset of a T-cell library whose sequence tags showed significant homology to other avian or mammalian sequences in the EMBL database. Placement of loci generated by Compton cDNA clones on the East Lansing linkage map contributes to development of a consensus from the two linkage maps and will help provide a framework to evaluate the phenotypic effects of these genes.
利用鸡的互补DNA（cDNA）探针，通过限制性片段长度多态性（RFLP）分析，在原鸡与白来航鸡回交基因定位参考群体的后代中对10个基因座进行定位。这些cDNA探针来自一个T细胞文库的子集，其序列标签在EMBL数据库中与其他禽类或哺乳动物序列具有显著同源性。康普顿cDNA克隆产生的基因座在东兰辛连锁图谱上的定位，有助于从这两个连锁图谱中形成一个共识，并将有助于提供一个框架来评估这些基因的表型效应。
