Coates D
Public Health Laboratory, Royal Preston Hospital, Preston, Lancashire, UK.
J Hosp Infect. 1996 Apr;32(4):283-94. doi: 10.1016/s0195-6701(96)90039-0.
The activity of sodium dichloroisocyanurate (NaDCC), peroxygen and glutaraldehyde disinfectants against spores of Bacillus subtilis NCTC 10073 was evaluated using suspension tests. The activity of aqueous solutions of NaDCC disinfectants increased with the level of available chlorine (av.Cl) but was considerably reduced by low levels of blood. Five percent Titan Sanitizer (1200 ppm av.Cl) achieved a > 10(5)-fold reduction in spore count (kill) in 3 h in the absence of blood but no kill in 3 h with 2% blood present. One percent Presept (3180 ppm av.Cl) achieved a > 10(5)-fold kill in 1 h in the absence of blood and in 2 h with 2% blood present. One percent Haz-Tab (5750 ppm av.Cl) achieved > 10(5)-fold kill in 5 min in the absence of blood and in 30 min with 2% blood present. One percent Virkon (peroxygen) achieved a 10(5)-fold kill in 2-3 h in the absence of blood but little kill in 3 h with 2% blood present. Nu-Cidex (peracetic acid) was rapid in action and tolerant of organic matter. A 24 h old solution achieved a > 10(5)-fold kill in 5 min with 10% serum present. Cidex Long-Life (glutaraldehyde) worked much slower; a 28-day-old solution achieved a > 10(5)-fold kill in 2 h with 4% blood present. Neat sporicidin (glutaraldehyde-phenate) was slightly superior to Cidex Long-Life but in a 1 in 8 dilution exhibited markedly reduced activity; 30-day-old solution achieved a 10(4)-fold kill in 10 h in the absence of blood but only a 10(2)-fold kill in 10 h with 2% blood present.
采用悬液试验评估了二氯异氰尿酸钠（NaDCC）、过氧化物和戊二醛消毒剂对枯草芽孢杆菌NCTC 10073孢子的活性。NaDCC消毒剂水溶液的活性随有效氯（av.Cl）水平的升高而增加，但低水平血液会使其活性大幅降低。5%的Titan消毒剂（有效氯1200 ppm）在无血液存在时3小时内使孢子数减少>10⁵倍（杀灭），但在有2%血液存在时3小时内无杀灭效果。1%的Presept（有效氯3180 ppm）在无血液存在时1小时内实现>10⁵倍杀灭，在有2%血液存在时2小时内实现>10⁵倍杀灭。1%的Haz-Tab（有效氯5750 ppm）在无血液存在时5分钟内实现>10⁵倍杀灭，在有2%血液存在时30分钟内实现>10⁵倍杀灭。1%的Virkon（过氧化物）在无血液存在时2 - 3小时内实现10⁵倍杀灭，但在有2%血液存在时3小时内杀灭效果甚微。Nu - Cidex（过氧乙酸）作用迅速且耐有机物。一份24小时的溶液在有10%血清存在时5分钟内实现>10⁵倍杀灭。Cidex Long - Life（戊二醛）作用慢得多；一份28天的溶液在有4%血液存在时2小时内实现>10⁵倍杀灭。纯的Sporicidin（戊二醛 - 酚盐）略优于Cidex Long - Life，但在1:8稀释时活性显著降低；一份30天的溶液在无血液存在时10小时内实现10⁴倍杀灭，但在有2%血液存在时10小时内仅实现10²倍杀灭。
