Gregory C R, Latimer K S, Niagro F D, Campagnoli R P, Steffens W L, Ritchie B W
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
J Vet Diagn Invest. 1996 Apr;8(2):151-5. doi: 10.1177/104063879600800202.
Eastern equine encephalomyelitis (F.EE) virus was detected in infected formalin-fixed horse and emu tissues and in infected chicken embryo fibroblasts. Results of in situ hybridization using a digoxigenin-labeled 40-base DNA probe complementary to a conserved region of the EEE virus RNA compared favorably with results of both virus isolation and serum neutralization tests. This technique may be useful for diagnosis of EEE virus infection in various animal species, especially when fresh tissues are not available for analysis, and also will provide a means for studying the involvement of alphaviruses in pathogenesis studies.
在感染的福尔马林固定马和鸸鹋组织以及感染的鸡胚成纤维细胞中检测到东部马脑脊髓炎（EEE）病毒。使用与EEE病毒RNA保守区域互补的地高辛标记40碱基DNA探针进行原位杂交的结果，与病毒分离和血清中和试验的结果相比具有优势。该技术可能有助于诊断各种动物物种中的EEE病毒感染，特别是在没有新鲜组织可供分析时，并且还将为研究甲病毒在发病机制研究中的作用提供一种手段。
