Schroeder-Tucker L, Wesley I V, Kiehlbauch J A, Larson D J, Thomas L A, Erickson G A
US Department of Agriculture, National Veterinary Services Laboratories, Ames, IA 50010, USA.
J Vet Diagn Invest. 1996 Apr;8(2):186-95. doi: 10.1177/104063879600800208.
Aerotolerant organisms resembling Campylobacter, now designated as Arcobacter, have been described from aborted farm animals and from cases of human enteritis worldwide. The goals of this study were 1) to attempt to recover Arcobacter spp. from cases of porcine abortion, 2) to characterize these isolates by phenotype and ribotype, and 3) to compare the usefulness of ribotype and phenotype patterns for identifying Arcobacter butzleri and the DNA hybridization groups 1A and 1B of A. cryaerophilus. Isolates of Arcobacter spp. from North Carolina and Iowa were recovered from porcine tissues. In Iowa, Arcobacter spp. were recovered from 43% (13/30) of porcine abortion cases evaluated. Isolations were made from placenta (44%), kidney (44%), and stomach contents (12%), which were the only tissues examined. The most reliable biochemical tests for A. butzleri included growth in 1% glycine and in 1.5% NaCl, weak catalase activity, and resistance to cadmium chloride. Arcobacter cryaerophilus strains were characterized by strong catalase activity and sensitivity to cadmium chloride. The DNA hybridization groups 1A and 1B of A. cryaerophilus could not be distinguished by biochemical tests. This represents the first description of A. cryaerophilus DNA group 1A in animals within the United States.
在世界各地，已从流产的农场动物和人类肠炎病例中发现了类似弯曲杆菌的耐氧微生物，现被命名为嗜冷栖热菌属。本研究的目的是：1）尝试从猪流产病例中分离出嗜冷栖热菌属；2）通过表型和核糖型对这些分离株进行特征描述；3）比较核糖型和表型模式在鉴定布氏嗜冷栖热菌以及嗜冷栖热菌DNA杂交组1A和1B方面的实用性。从北卡罗来纳州和爱荷华州的猪组织中分离出嗜冷栖热菌属菌株。在爱荷华州，在所评估的30例猪流产病例中，有43%（13/30）分离出嗜冷栖热菌属。分离样本来自胎盘（44%）、肾脏（44%）和胃内容物（12%），这些是仅有的检测组织。对布氏嗜冷栖热菌最可靠的生化检测包括在1%甘氨酸和1.5%氯化钠中生长、弱过氧化氢酶活性以及对氯化镉的抗性。嗜冷栖热菌菌株的特征是过氧化氢酶活性强且对氯化镉敏感。嗜冷栖热菌的DNA杂交组1A和1B无法通过生化检测区分。这是美国境内动物中首次对嗜冷栖热菌DNA组1A的描述。
