Rota S, Yildiz A, Kuştimur S, Akbaş E, Günay A, Güner H
Department of Microbiology, Gazi University Medical School, Beşevler, Ankara, Turkey.
Int J Gynaecol Obstet. 1995 Dec;51(3):225-8.
Chlamydia trachomatis is an important etiological agent in female genital infection and may result in infertility. In recent years rapid diagnostic methods have become widely used as alternatives to cell culture. Our objective was to evaluate the technique of direct fluorescence assay (DFA) in estimating the presence of C. trachomatis.
Specimens taken from 40 infertile and 20 fertile women were examined by DFA for the presence of C. trachomatis.
Six of forty (15%) infertile women were found to be positive whereas no positive specimens were detected in the control group. When the specimens were grouped into those which were adequate or inadequate, 19 and five specimens, respectively, were adequate in the infertile and healthy groups. If only adequate specimens are included in estimating the presence of Chlamydia, the percentage is 31.6%.
In order to use DFA as a more reliable and rapid diagnostic test of C. trachomatis in female genital infection, false-negativity must be eliminated. Specimens must be collected adequately and concentrated in order to achieve optimal diagnostic success.
沙眼衣原体是女性生殖器感染的重要病原体，可能导致不孕。近年来，快速诊断方法已被广泛用作细胞培养的替代方法。我们的目的是评估直接荧光抗体法（DFA）在检测沙眼衣原体存在方面的技术。
对40名不孕女性和20名生育期女性的样本进行DFA检测，以确定是否存在沙眼衣原体。
40名不孕女性中有6名（15%）检测结果呈阳性，而对照组未检测到阳性样本。当将样本分为足够或不足两类时，不孕组和健康组分别有19份和5份样本足够。如果仅将足够的样本用于评估衣原体的存在，百分比为31.6%。
为了将DFA用作女性生殖器感染中沙眼衣原体更可靠、快速的诊断测试，必须消除假阴性。必须充分收集和浓缩样本，以实现最佳诊断效果。
