Kunkle R A, Steadham E M, Cheville N F
National Animal Disease Center, United States Department of Agriculture, Ames, IA 50010, USA.
Vet Immunol Immunopathol. 1995 Dec;49(3):271-9. doi: 10.1016/0165-2427(95)05464-2.
T-lymphocyte subpopulations were examined in vivo by computer-assisted morphometry of superficial cervical lymph nodes of cattle vaccinated with Brucella abortus. Twenty-four 8-month-old Hereford heifers were injected subcutaneously in the axillary area with 1 x 10(10) live B. abortus strain RB51 (SRB51, n = 12) or strain 19 (S19, n = 6) suspended in 2 ml of saline. Six control heifers were injected with sterile saline. Lymph nodes were collected at 1, 2, 4, 6, 10 and 12 weeks postvaccination. Both SRB51 and S19 were cultured from lymph nodes, but SRB51 persisted for a longer period after vaccination (10 weeks) than S19 (6 weeks). Cryostat sections were incubated with monoclonal antibody to CD4 (IL-A11), CD8 (IL-A51), or gamma/delta (IL-A29) bovine T-cell surface antigen and processed for immunoperoxidase staining. Numbers of stained lymphocytes in randomly selected fields were calculated using image-analysis software. There were no significant differences in the number (P = 0.07) or relative proportions (P = 0.22) of CD4+, CD8+, and gamma/delta+ lymphocytes in SRB51, S19, and control lymph nodes. There was a statistically significant difference in the distribution of the three T-cell subsets (P = 0.001). The CD4+ cells were most closely grouped and the gamma/delta+ cells had the most widely scattered distribution, regardless of vaccination status. The results support other studies indicating lymphocyte depletion is not a sequela of infection with B. abortus vaccine strains given to conventionally reared cattle.
通过计算机辅助形态测量法对接种流产布鲁氏菌的牛的颈浅淋巴结进行体内T淋巴细胞亚群检测。24头8月龄的赫里福德小母牛在腋窝区域皮下注射1×10(10)个活的流产布鲁氏菌RB51菌株（SRB51，n = 12）或19菌株（S19，n = 6），菌株悬浮于2毫升盐水中。6头对照小母牛注射无菌盐水。在接种疫苗后1、2、4、6、10和12周收集淋巴结。SRB51和S19均能从淋巴结中培养出来，但SRB51在接种疫苗后持续存在的时间（10周）比S19（6周）更长。将冰冻切片与抗牛CD4（IL-A11）、CD8（IL-A51）或γ/δ（IL-A29）T细胞表面抗原的单克隆抗体孵育，并进行免疫过氧化物酶染色处理。使用图像分析软件计算随机选择视野中染色淋巴细胞的数量。SRB51、S19和对照淋巴结中CD4+、CD8+和γ/δ+淋巴细胞的数量（P = 0.07）或相对比例（P = 0.22）无显著差异。三种T细胞亚群的分布存在统计学显著差异（P = 0.001）。无论接种状态如何，CD4+细胞聚集最紧密，γ/δ+细胞分布最分散。这些结果支持了其他研究，表明淋巴细胞耗竭不是常规饲养的牛接种流产布鲁氏菌疫苗株感染后的后遗症。
