Dorneles Elaine M S, Teixeira-Carvalho Andréa, Araújo Márcio S S, Lima Graciela Kunrath, Martins-Filho Olindo A, Sriranganathan Nammalwar, Lage Andrey P
Laboratório de Bacteriologia Aplicada, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brazil.
Vaccine. 2014 Oct 21;32(46):6034-8. doi: 10.1016/j.vaccine.2014.08.060. Epub 2014 Sep 10.
The aims of this study were to address the protective immune response induced by S19 vaccination (n=10) and RB51 revaccination, in pregnant (n=9) and non-pregnant (n=10) S19 calfhood-vaccinated cattle as follows: evaluate the in vitro CD4(+) and CD8(+) T-lymphocytes specific proliferation, and in vitro expression of IFN-γ by CD4(+) and CD8(+) T-cells and IL-4 by CD4(+), CD8(+) and CD21(+) lymphocytes subset. Upon in vitro stimulation with γ-irradiated Brucella abortus 2308, blood mononuclear cells from S19 vaccinated and RB51 revaccinated cows exhibited significantly higher proliferation of CD4(+) and CD8(+) T-lymphocytes and CD4(+)IFN-γ(+) T-cells compared to non-vaccinated animals. RB51 revaccination, regardless of the pregnancy status, did not enhance the proliferation of CD4(+) or CD8(+) T-cells nor IFN-γ or IL-4 production. Data from the present study suggest that cattle's cellular immune response induced after brucellosis vaccination and revaccination is due to CD4(+) and CD8(+) T-lymphocytes, being CD4(+) T-cells the main source of IFN-γ.
本研究的目的是探讨S19疫苗接种（n = 10）和RB51再接种对犊牛期接种过S19疫苗的怀孕（n = 9）和未怀孕（n = 10）母牛所诱导的保护性免疫反应，具体如下：评估体外CD4(+)和CD8(+) T淋巴细胞的特异性增殖，以及CD4(+)和CD8(+) T细胞体外IFN-γ的表达和CD4(+)、CD8(+)及CD21(+)淋巴细胞亚群体外IL-4的表达。在用γ射线照射的流产布鲁氏菌2308进行体外刺激后，与未接种疫苗的动物相比，接种S19疫苗和再接种RB51疫苗的母牛的血液单核细胞表现出CD4(+)和CD8(+) T淋巴细胞以及CD4(+)IFN-γ(+) T细胞的显著更高增殖。无论怀孕状态如何，RB51再接种均未增强CD4(+)或CD8(+) T细胞的增殖，也未增强IFN-γ或IL-4的产生。本研究数据表明，布鲁氏菌病疫苗接种和再接种后诱导的牛细胞免疫反应归因于CD4(+)和CD8(+) T淋巴细胞，其中CD4(+) T细胞是IFN-γ的主要来源。
