Sugiyama S, Yamada K, Ozawa T
Department of Biomedical Chemistry, University of Nagoya, Showa-ku, Japan.
Biochem Mol Biol Int. 1995 Dec;37(6):1111-20.
Pharmacological intervention was investigated into the age-associated decline in mitochondrial function. Rats aged 7 weeks were divided into two groups; the control group (standard diet), and the coenzyme Q10 group (fed with 0.2% coenzyme Q10 diet). Mitochondria from skeletal muscle (psoai major) and cardiac muscle were prepared from rats aged 7, 35, and 55 weeks, and enzymic activities were measured in four complexes of the mitochondrial electron transport chain. Age-associated declines in activities of complexes I and IV of psoai major were significantly mitigated in the coenzyme Q10 group. The activities of complexes I and IV of heart mitochondria did not change significantly throughout the experiment in either group. Moreover, aging up to 55 weeks had no significant effect on activities of complexes II and III in either tissue. It is concluded that intake of a coenzyme Q10 rich diet might retard the normally observed age-associated decline in overall mitochondrial respiratory function in rat skeletal muscle.
研究了针对与年龄相关的线粒体功能衰退的药物干预措施。将7周龄的大鼠分为两组：对照组（标准饮食）和辅酶Q10组（喂食含0.2%辅酶Q10的饮食）。从7周龄、35周龄和55周龄的大鼠中制备骨骼肌（腰大肌）和心肌的线粒体，并在线粒体电子传递链的四个复合物中测量酶活性。在辅酶Q10组中，腰大肌复合物I和IV活性与年龄相关的下降得到显著缓解。在整个实验过程中，两组中心肌线粒体复合物I和IV的活性均无显著变化。此外，在55周龄之前，两种组织中复合物II和III的活性均未受到衰老的显著影响。结论是，摄入富含辅酶Q10的饮食可能会延缓大鼠骨骼肌中通常观察到的与年龄相关的整体线粒体呼吸功能衰退。
