科威特1型胰岛素依赖型糖尿病患者及其一级亲属的淋巴细胞亚群异常。

Abnormal lymphocyte subsets in Kuwaiti patients with type-1 insulin-dependent diabetes mellitus and their first-degree relatives.

作者信息

Kaaba S A, Al-Harbi S A

机构信息

Department of Medical Laboratory Technology, Faculty of Allied Health Sciences and Nursing, Kuwait University, Kuwait.

出版信息

Immunol Lett. 1995 Sep;47(3):209-13. doi: 10.1016/0165-2478(95)00088-5.

DOI:10.1016/0165-2478(95)00088-5

PMID:8747721

Abstract

Circulating lymphocyte subset imbalance is associated with type-1 insulin-dependent diabetes mellitus (IDDM). To examine the imbalance in these immunoregulatory cells in Kuwaitis with type-1 diabetes and their first-degree relatives we analysed T-lymphocyte subsets and HLA-DR expression (activation) in 18 IDDM patients with a family history of IDDM and 18 non-diabetic first-degree relatives of the IDDM patients. Both IDDM patients and their first-degree relatives showed a mild lymphopenia. Total T lymphocytes, CD3+ cells, in IDDM patients and their first-degree relatives were reduced compared to control subjects (P < 0.001). Total B lymphocytes, CD19+ cells, was increased in IDDM patients (P = 0.001), but was comparable to controls in IDDM patients' first-degree relatives. No quantitative abnormality was demonstrated in CD4+ cells in IDDM patients; however, these cells were higher in their first-degree relatives (P = 0.0089). Suppressor T lymphocytes, CD8+ cells, in first-degree relatives and controls were not significantly different; however, these cells were significantly reduced in IDDM patients (P = 0.001). The ratio of CD4+/CD8+ cells was higher in IDDM patients and their first-degree relatives compared to controls (P = 0.0007 and 0.0103, respectively). Activated T lymphocytes, HLA-DR+ CD3+ cells, were significantly increased in IDDM patients and their first-degree relatives. HLA-DR3 was the most common antigen found in IDDM patients (77% vs. 20% in controls, P = 0.00021). The second most common antigen was HLA-DR4 (55% vs. 24% in controls, P = 0.0566). However, no relationship was found in the levels of CD3+, CD4+ or CD8+ cells in patients possessing either DR3 or DR4. These results suggest that T-lymphocyte subset imbalance not only characterizes the cellular autoimmunity in the pathogenesis of IDDM but may also be significant in early pre-diabetic stages in those with a family history of IDDM.

摘要

循环淋巴细胞亚群失衡与1型胰岛素依赖型糖尿病（IDDM）相关。为了研究科威特1型糖尿病患者及其一级亲属中这些免疫调节细胞的失衡情况，我们分析了18名有IDDM家族史的IDDM患者和18名IDDM患者的非糖尿病一级亲属的T淋巴细胞亚群及HLA - DR表达（激活情况）。IDDM患者及其一级亲属均表现出轻度淋巴细胞减少。与对照组相比，IDDM患者及其一级亲属的总T淋巴细胞（CD3 +细胞）减少（P < 0.001）。IDDM患者的总B淋巴细胞（CD19 +细胞）增加（P = 0.001），但其一级亲属中的总B淋巴细胞与对照组相当。IDDM患者的CD4 +细胞未显示出数量异常；然而，这些细胞在其一级亲属中更高（P = 0.0089）。一级亲属和对照组中的抑制性T淋巴细胞（CD8 +细胞）无显著差异；然而，IDDM患者中的这些细胞显著减少（P = 0.001）。与对照组相比，IDDM患者及其一级亲属的CD4 + / CD8 +细胞比值更高（分别为P = 0.0007和0.0103）。IDDM患者及其一级亲属中活化的T淋巴细胞（HLA - DR + CD3 +细胞）显著增加。HLA - DR3是IDDM患者中最常见的抗原（77%，对照组为20%，P = 0.00021）。第二常见的抗原是HLA - DR4（55%，对照组为24%，P = 0.0566）。然而，携带DR3或DR4的患者中，CD3 +、CD4 +或CD8 +细胞水平未发现相关性。这些结果表明，T淋巴细胞亚群失衡不仅是IDDM发病机制中细胞自身免疫的特征，而且在有IDDM家族史的糖尿病前期早期阶段可能也具有重要意义。