Magrassi L, Butti G, Pezzotta S, Infuso L, Milanesi G
Department of Surgery, University of Pavia, IRCCS Policlinico S. Mattco, Italy.
Acta Neurochir (Wien). 1995;133(3-4):184-90. doi: 10.1007/BF01420072.
The biological significance of vitamin D receptors expressed by glioblastoma and other glial tumours is still unclear. In an effort to clarify this issue we studied the effects of increasing concentrations of 25-dihydroxyvitamin D3 and its metabolite 1 alpha,25-dihydroxyvitamin D3 on two human glioblastoma cell lines. Both substances were capable of inducing a significant (> 50%) reduction in growth of the two glioblastoma cell lines at dosages over 5 microM. When the HU 70 cell line was treated by increasing dilutions of 25-dihydroxyvitamin D3 combined with 1 microM all trans-retinoic acid, significant inhibition was apparent even after addition of 25-dihydroxyvitamin D3 in the nanomolar range. Reduction of growth index was mainly due to induced cell death. Our results provide in vitro evidence that vitamin D metabolites alone or in combination with retinoids may be potentially useful agents in the differentiation therapy of human malignant gliomas.
胶质母细胞瘤和其他神经胶质瘤所表达的维生素D受体的生物学意义仍不清楚。为了阐明这个问题，我们研究了浓度不断增加的25-二羟基维生素D3及其代谢物1α,25-二羟基维生素D3对两个人类胶质母细胞瘤细胞系的影响。两种物质在剂量超过5微摩尔时均能够使两种胶质母细胞瘤细胞系的生长显著降低（>50%）。当用25-二羟基维生素D3的递增稀释液联合1微摩尔全反式维甲酸处理HU 70细胞系时，即使加入纳摩尔范围的25-二羟基维生素D3也会出现明显的抑制作用。生长指数的降低主要是由于诱导细胞死亡。我们的结果提供了体外证据，表明单独的维生素D代谢物或与类维生素A联合使用可能是人类恶性胶质瘤分化治疗中的潜在有用药物。
