来自酿酒酵母第六条染色体的一个18.8 kb片段的测序。

Sequencing of an 18.8 kb fragment from Saccharomyces cerevisiae chromosome VI.

作者信息

Naitou M, Ozawa M, Sasanuma S, Kobayashi M, Hagiwara H, Shibata T, Hanaoka F, Watanabe K, Ono A, Yamazaki M

机构信息

Division of Human Genome Research, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.

出版信息

Yeast. 1995 Dec;11(15):1525-32. doi: 10.1002/yea.320111508.

DOI:10.1002/yea.320111508

PMID:8750241

Abstract

The nucleotide sequence of lambda phage clone 4121, which contains the 18.8 kb fragment of Saccharomyces cerevisiae chromosome VI left arm, was determined. This sequence had seven open reading frames (ORFs), four of which were identical to known genes (ACT1, YPT1, TUB2 and RPO41). Another three ORFs (4121orfR003, 4121orfR004 and 4121orfRN001) were highly homologous to FET3 multi-copper oxidase, glucose transport protein, and hypothetical protein of YIL106w on chromosome IX, respectively. 4121orfRN01 is suggested to contain an intron.

摘要

测定了λ噬菌体克隆4121的核苷酸序列，该克隆包含酿酒酵母VI号染色体左臂的18.8 kb片段。该序列有七个开放阅读框（ORF），其中四个与已知基因（ACT1、YPT1、TUB2和RPO41）相同。另外三个开放阅读框（4121orfR003、4121orfR004和4121orfRN001）分别与FET3多铜氧化酶、葡萄糖转运蛋白以及IX号染色体上YIL106w的假定蛋白高度同源。4121orfRN01被认为含有一个内含子。