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对酿酒酵母七号染色体左臂上一个40.5 kb片段进行测序。

Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae.

作者信息

Coglievina M, Klima R, Bertani I, Delneri D, Zaccaria P, Bruschi C V

机构信息

Microbiology Group, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.

出版信息

Yeast. 1997 Jan;13(1):55-64. doi: 10.1002/(SICI)1097-0061(199701)13:1<55::AID-YEA48>3.0.CO;2-9.

Abstract

The nucleotide sequence of a 40.5 kb DNA fragment from the left arm of chromosome VII of Saccharomyces cerevisiae was determined and analysed. Twenty-eight open reading frames (ORFs) longer than 300 nucleotides were identified. Eight of the them correspond to the following known yeast genes: EMP24, GCN1, SPO8, COX13, CDC55, RPS26, COX4 and LSR1, also called GTS1. Twelve ORFs are new, among them eight show homology with other genes while four have no homology with any sequence in the databases. Eight additional ORFs are internal to or partially overlapping with other ORFs.

摘要

测定并分析了来自酿酒酵母七号染色体左臂的一个40.5 kb DNA片段的核苷酸序列。鉴定出28个长度超过300个核苷酸的开放阅读框(ORF)。其中8个对应于以下已知的酵母基因:EMP24、GCN1、SPO8、COX13、CDC55、RPS26、COX4和LSR1(也称为GTS1)。12个ORF是新的,其中8个与其他基因具有同源性,而4个与数据库中的任何序列都没有同源性。另外8个ORF位于其他ORF内部或与之部分重叠。

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