el-Gamal Y, Hossny E, Mabrouk R, el-Gamasey T
Department of Pediatrics, Ain Shams University, Cairo, Egypt.
Pediatr Allergy Immunol. 1995 Aug;6(3):165-9. doi: 10.1111/j.1399-3038.1995.tb00277.x.
The antiperinuclear factor (APF) was estimated by immunofluorescent microscopy in the sera of 32 children and adolescents with juvenile rheumatoid arthritis (JRA) in comparison to a group of 16 children and adolescents with other rheumatologic disorders and a group of 20 age-matched healthy subjects. The APF was detected in 17 children with JRA (53%), in only one patient in the group of other rheumatologic disorders (6%), and in 2 healthy children (10%). Accordingly, APF had a sensitivity of 53%, a specificity of 92%, and a diagnostic efficiency of 74% in our series. APF was found to have a higher diagnostic gain in rheumatoid factor (RF) seronegative cases than did the RF in APF negative cases, meaning a higher sensitivity of APF as compared to the RF. The APF seropositivity was neither altered by the use of corticosteroids nor influenced by the age, gender, duration of illness, or number of joints affected. Three out of 5 patients with JRA had the APF detected in their synovial fluid; they were running rather a severe course of illness. The use of the APF could be an aid in the diagnosis of JRA.
采用免疫荧光显微镜法对32例青少年类风湿性关节炎（JRA）患儿及青少年的血清抗核周因子（APF）进行检测，并与16例患有其他风湿性疾病的儿童及青少年以及20例年龄匹配的健康受试者进行比较。在17例JRA患儿（53%）中检测到APF，在其他风湿性疾病组中仅1例患者（6%）检测到APF，2例健康儿童（10%）检测到APF。因此，在我们的研究系列中，APF的敏感性为53%，特异性为92%，诊断效率为74%。发现APF在类风湿因子（RF）血清阴性病例中的诊断价值高于RF在APF阴性病例中的诊断价值，这意味着与RF相比，APF具有更高的敏感性。APF血清阳性既不受皮质类固醇使用的影响，也不受年龄、性别、病程或受累关节数量的影响。5例JRA患者中有3例在其滑液中检测到APF；他们的病情进展较为严重。APF的检测有助于JRA的诊断。
