Ramig R F, Cross R K, Fields B N
J Virol. 1977 Jun;22(3):726-33. doi: 10.1128/JVI.22.3.726-733.1977.
The virus-specific double-stranded genome RNA and polypeptides present in virions and cells infected with the three mammalian reovirus serotypes have been examined by co-electrophoresis in several different polyacrylamide gel systems. The double-stranded RNA and polypeptide species previously described for type 3 Dearing were found to have corresponding species in the other serotypes examined. In each serotype several RNA and polypeptide species were found to have different electrophoretic mobilities from the corresponding RNA or polypeptide species of type 3 Dearing. The combination of electrophoretic variants among the RNAs and polypeptides of the reovirus serotypes gave electrophoretic markers in all 10 of the reovirus genes. The usefulness of these electrophoretic markers in "mapping" the reovirus genome is discussed.
通过在几种不同的聚丙烯酰胺凝胶系统中进行共电泳，对感染三种哺乳动物呼肠孤病毒血清型的病毒粒子和细胞中存在的病毒特异性双链基因组RNA和多肽进行了检测。先前描述的3型迪尔林病毒的双链RNA和多肽种类在其他检测的血清型中也有相应的种类。在每种血清型中，发现几种RNA和多肽种类的电泳迁移率与3型迪尔林病毒相应的RNA或多肽种类不同。呼肠孤病毒血清型的RNA和多肽中的电泳变体组合在呼肠孤病毒的所有10个基因中都给出了电泳标记。讨论了这些电泳标记在呼肠孤病毒基因组“图谱绘制”中的用途。
