Melton T, Stoneking M
Department of Anthropology, Pennsylvania State University, University Park, PA., USA.
J Forensic Sci. 1996 Jul;41(4):591-602.
Variation in the mitochondrial DNA (mtDNA) control region as detected by sequence-specific oligonucleotide (SSO) probes is described for 993 individuals in 11 ethnic Asian populations. Estimates of diversity for mtDNA types exceed 0.94 in all populations, while 53% of the 255 types that were observed occur only once. Of 96 shared types, four occur at frequencies of greater than 10% but less than 17% in any one population. There is statistically significant heterogeneity among these 11 populations, however, an analysis of variance incorporating genetic distances between types shows that at least 95% of the variation present in the total sample is attributable to within-population diversity, while only 5% is due to between-population differences. Overall, heterogeneity with respect to mtDNA SSO types is grossly correlated with geographic distance between populations; the most extreme heterogeneity was observed between populations from East Asia and populations from West Asia. With respect to population genetics, the control region of mtDNA exhibits satisfactory qualities as a DNA typing locus.
采用序列特异性寡核苷酸（SSO）探针检测了11个亚洲民族群体中993名个体线粒体DNA（mtDNA）控制区的变异情况。所有群体中mtDNA类型的多样性估计值均超过0.94，而观察到的255种类型中有53%仅出现一次。在96种共享类型中，有4种在任何一个群体中的出现频率都大于10%但小于17%。这11个群体之间存在统计学上显著的异质性，然而，一项纳入类型间遗传距离的方差分析表明，总样本中至少95%的变异归因于群体内部的多样性，而仅有5%是由于群体间的差异。总体而言，mtDNA SSO类型的异质性与群体间的地理距离高度相关；东亚群体与西亚群体之间观察到的异质性最为极端。就群体遗传学而言，mtDNA控制区作为一个DNA分型位点表现出令人满意的特性。
